Construct: ORF TRCN0000472474
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008739.1_s317c1
- Derived from:
- ccsbBroadEn_06244
- DNA Barcode:
- GATAAATCTGAACAACTCAACATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GABRD (2563)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472474
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2563 | GABRD | gamma-aminobutyric acid typ... | NM_000815.5 | 99.9% | 100% | 330T>C |
| 2 | human | 2563 | GABRD | gamma-aminobutyric acid typ... | XM_011541194.3 | 94.8% | 90.7% | (many diffs) |
| 3 | human | 2563 | GABRD | gamma-aminobutyric acid typ... | XM_017000936.1 | 64.4% | 63.2% | (many diffs) |
| 4 | mouse | 14403 | Gabrd | gamma-aminobutyric acid (GA... | NM_008072.2 | 85.3% | 92% | (many diffs) |
| 5 | mouse | 14403 | Gabrd | gamma-aminobutyric acid (GA... | XM_006538546.3 | 79.4% | 85.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1422
- ORF length:
- 1356
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgcgcccgcc cggctgctgg ccccgctcct gctcctctgc gcgcagcagc 121 tccgcggcac cagagcgatg aatgacatcg gcgactacgt gggctccaac ctggagatct 181 cctggctccc caacctggac gggctgatag ccggctacgc ccgcaacttc cggcctggca 241 tcggaggccc ccccgtgaat gtggcccttg ccctggaggt ggccagcatc gaccacatct 301 cagaggccaa catggagtac accatgacgg tgttcctgca ccagagctgg cgggacagca 361 ggctctccta caaccacacc aacgagaccc tgggcctgga cagccgcttc gtggacaagc 421 tgtggctgcc cgacaccttc atcgtgaacg ccaagtcggc ctggttccac gacgtgacgg 481 tggagaacaa gctcatccgg ctgcagcccg acggcgtgat cctgtacagc atccgaatca 541 cctccactgt ggcctgcgac atggacctgg ccaaataccc catggacgag caggagtgca 601 tgctggacct ggagagctac ggttactcat cggaggacat cgtctactac tggtcggaga 661 gccaggagca catccacggg ctggacaagc tgcagctggc gcagttcacc atcaccagct 721 accgcttcac cacggagctg atgaacttca agtccgctgg ccagttccca cggctcagcc 781 tgcacttcca cctgcggagg aaccgcggcg tgtacatcat ccaatcctac atgccctccg 841 tcctgctggt cgccatgtcc tgggtctcct tctggatcag ccaggcggcg gtgcccgcca 901 gggtgtctct aggcatcacc acggtgctga cgatgaccac gctcatggtc agtgcccgcT 961 CCTCCCTGCC ACGGGCATCA GCCATCAAGG CACTGGACGT CTACTTCTGG ATCTGCTATG 1021 TCTTCGTGTT TGCCGCCCTG GTGGAGTACG CCTTTGCTCA TTTCAACGCC GACTACAGGA 1081 AGAAGCAGAA GGCCAAGGTC AAGGTCTCCA GGCCGAGGGC AGAGATGGAC GTGAGGAACG 1141 CCATTGTCCT CTTCTCCCTC TCTGCTGCCG GCGTCACGCA GGAGCTGGCC ATCTCCCGCC 1201 GGCAGCGCCG CGTCCCGGGG AACCTGATGG GCTCCTACAG GTCGGTGGGG GTGGAGACAG 1261 GGGAGACGAA GAAGGAGGGG GCAGCCCGCT CAGGAGGCCA GGGGGGCATC CGTGCCCGGC 1321 TCAGGCCCAT CGACGCAGAC ACCATTGACA TTTACGCCCG CGCTGTGTTC CCTGCGGCGT 1381 TTGCGGCCGT CAATGTCATC TACTGGGCGG CATACGCCAT GTGCCCAACT TTCTTGTACA 1441 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1501 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1561 AGGACGAGAT AAATCTGAAC AACTCAACAT TACGCGTTAA GTCgacaatc aacctctgga 1621 ttacaaaatt tgtgaaagat t