Construct: ORF TRCN0000472490
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001328.1_s317c1
- Derived from:
- ccsbBroadEn_11585
- DNA Barcode:
- TCGATTGCACTATGTTAGAATGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPSF6 (11052)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472490
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11052 | CPSF6 | cleavage and polyadenylatio... | NM_007007.3 | 86.6% | 86.7% | 354G>A;561_779del;1467A>G |
| 2 | human | 11052 | CPSF6 | cleavage and polyadenylatio... | XM_005268590.3 | 86.4% | 86.5% | 354G>A;561_779del;1467_1470delAAGAinsG |
| 3 | human | 11052 | CPSF6 | cleavage and polyadenylatio... | NM_001300947.2 | 81.1% | 81.2% | 354G>A;561_890del;1578A>G |
| 4 | human | 11052 | CPSF6 | cleavage and polyadenylatio... | XM_005268588.3 | 81% | 81.1% | 354G>A;561_890del;1578_1581delAAGAinsG |
| 5 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | NM_001013391.2 | 80% | 86.5% | (many diffs) |
| 6 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513858.3 | 80% | 86.5% | (many diffs) |
| 7 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | NM_001310609.1 | 79.8% | 86.4% | (many diffs) |
| 8 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513856.3 | 79.8% | 86.4% | (many diffs) |
| 9 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513854.3 | 75% | 81.1% | (many diffs) |
| 10 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513855.2 | 75% | 81.1% | (many diffs) |
| 11 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513851.3 | 74.8% | 80.9% | (many diffs) |
| 12 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513852.2 | 74.8% | 80.9% | (many diffs) |
| 13 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XM_006513853.2 | 74.8% | 80.9% | (many diffs) |
| 14 | mouse | 432508 | Cpsf6 | cleavage and polyadenylatio... | XR_001779551.1 | 10.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1500
- ORF length:
- 1434
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacggcgtg gaccacatag acatttacgc ggatgtcggc gaagagttca 121 accaggaagc tgaatatggt gggcatgatc agatagattt gtatgacgat gtcatatctc 181 catctgcaaa taatggagat gccccagaag accgagatta catggatact ctcccaccaa 241 ctgttggtga tgatgtgggt aaaggagcag caccaaatgt tgtctataca tatactggaa 301 agagaattgc attatatatt ggaaatctaa catggtggac aacagatgaa gacttaactg 361 aagcagttca ttctttggga gtaaatgata ttttggagat aaaatttttt gaaaatcgag 421 caaatggcca gtcaaagggg tttgcccttg ttggtgttgg atctgaagca tcttcaaaaa 481 agttaatgga tctgttacct aaaagagaac ttcatggtca gaatcctgtt gtaactccat 541 gcaataaaca gttcctgagt caatttgaaa tgcagtccag gaaaactaca caatcaggac 601 aaatgtctgg ggaaggtaaa gctggtcctc ctctagctgg gcctcctaat cgaggagatc 661 gccctccacc accagttctt tttcctggac aaccttttgg gcagcctcca ttgggtccac 721 ttcctcctgg ccctccacct ccagttccag gctacggccc ccctcctggc ccaccacctc 781 cacaacaggg accacctcca cctccaggcc cctttccacc tcgtccaccc ggtccacttg 841 ggccacccct tacactagct cctcctccgc atcttcctgg accacctcca ggtgccccac 901 cgccagctcc gcatgtgaac ccagctttct ttcctccacc aactaacagt ggcatgccta 961 catcagatag ccgaggtcca ccaccaacag atccatatgg gcgacctcca ccatatgata 1021 ggggtgacta tggcccccct ggaagggaaa tgGATACTGC AAGAACGCCA TTGAGTGAAG 1081 CTGAATTTGA AGAAATCATG AATAGAAATA GGGCAATCTC AAGCAGTGCT ATTTCGAGAG 1141 CTGTGTCTGA TGCCAGTGCT GGTGATTATG GGAGTGCTAT TGAGACACTG GTAACTGCAA 1201 TTTCTTTAAT TAAACAATCC AAAGTATCTG CTGATGATCG TTGCAAAGTT CTTATTAGTT 1261 CTTTGCAAGA TTGCCTTCAT GGAATTGAGT CCAAGTCTTA TGGTTCTGGA TCGAGACGTG 1321 AACGATCAAG AGAGAGGGAC CATAGTAGAT CACGAGAAAA GAGTCGACGT CATAAATCCC 1381 GTAGTAGAGA CCGTCATGAC GATTATTACA GAGAGAGAAG CAGAGAACGA GAGAGGCACC 1441 GGGATCGTGA CCGAGACCGT GACCGAGAGC GTGACCGAGA GCGCGAATAT CGTCATCGTT 1501 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1561 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1621 TTTATATATC TTGTGGAAAG GACGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT 1681 CGATTGCACT ATGTTAGAAT GATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 1741 tttgtgaaag att