Construct: ORF TRCN0000472498
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019044.2_s317c1
- Derived from:
- ccsbBroadEn_14628
- DNA Barcode:
- CTTTCTAGATTTTGCCGGTGTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHB1 (2047)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472498
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2047 | EPHB1 | EPH receptor B1 | NM_004441.5 | 100% | 100% | |
| 2 | human | 2047 | EPHB1 | EPH receptor B1 | XM_017005866.2 | 94.7% | 94.7% | 1297_1458del |
| 3 | human | 2047 | EPHB1 | EPH receptor B1 | XM_017005867.1 | 93.2% | 92.8% | (many diffs) |
| 4 | human | 2047 | EPHB1 | EPH receptor B1 | XM_024453389.1 | 92.6% | 92.6% | 0_1ins66;1231_1392del |
| 5 | human | 2047 | EPHB1 | EPH receptor B1 | XM_024453390.1 | 92.6% | 92.6% | 0_1ins66;1231_1392del |
| 6 | mouse | 270190 | Ephb1 | Eph receptor B1 | NM_173447.3 | 91.3% | 98.8% | (many diffs) |
| 7 | mouse | 270190 | Ephb1 | Eph receptor B1 | NM_001168296.1 | 87.3% | 94.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3021
- ORF length:
- 2952
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccctggat tatctactac tgctcctcct ggcatccgca gtggctgcga 121 tggaagaaac gttaatggac accagaacgg ctactgcaga gctgggctgg acggccaatc 181 ctgcgtccgg gtgggaagaa gtcagtggct acgatgaaaa cctgaacacc atccgcacct 241 accaggtgtg caatgtcttc gagcccaacc agaacaattg gctgctcacc accttcatca 301 accggcgggg ggcccatcgc atctacacag agatgcgctt cactgtgaga gactgcagca 361 gcctccctaa tgtcccagga tcctgcaagg agaccttcaa cttgtattac tatgagactg 421 actctgtcat tgccaccaag aagtcagcct tctggtctga ggccccctac ctcaaagtag 481 acaccattgc tgcagatgag agcttctccc aggtggactt tgggggaagg ctgatgaagg 541 taaacacaga agtcaggagc tttgggcctc ttactcggaa tggtttttac ctcgcttttc 601 aggattatgg agcctgtatg tctcttcttt ctgtccgtgt cttcttcaaa aagtgtccca 661 gcattgtgca aaattttgca gtgtttccag agactatgac aggggcagag agcacatctc 721 tggtgattgc tcggggcaca tgcatcccca acgcagagga agtggacgtg cccatcaaac 781 tctactgcaa cggggatggg gaatggatgg tgcctattgg gcgatgcacc tgcaagcctg 841 gctatgagcc tgagaacagc gtggcatgca aggcttgccc tgcagggaca ttcaaggcca 901 gccaggaagc tgaaggctgc tcccactgcc cctccaacag ccgctcccct gcagaggcgt 961 ctcccatctg cacctgtcgg accggttatt accgagcgga ctttgaccct ccagaagtgg 1021 catgcactag cgtcccatca ggtccccgca atgttatctc catcgtcaat gagacgtcca 1081 tcattctgga gtggcaccct ccaagggaga caggtgggcg ggatgatgtg acctacaaca 1141 tcatctgcaa aaagtgccgg gcagaccgcc ggagctgctc ccgctgtgac gacaatgtgg 1201 agtttgtgcc caggcagctg ggcctgacgg agtgccgcgt ctccatcagc agcctgtggg 1261 cccacacccc ctacaccttt gacatccagg ccatcaatgg agtctccagc aagagtccct 1321 tccccccaca gcacgtctct gtcaacatca ccacaaacca agccgccccc tccaccgttc 1381 ccatcatgca ccaagtcagt gccactatga ggagcatcac cttgtcatgg ccacagccgg 1441 agcagcccaa tggcatcatc ctggactatg agatccggta ctatgagaag gaacacaatg 1501 agttcaactc ctccatggcc aggagtcaga ccaacacagc aaggattgat gggctgcggc 1561 ctggcatggt atatgtggta caggtgcgtg cccgcactgt tgctggctac ggcaagttca 1621 gtggcaagat gtgcttccag actctgactg acgatgatta caagtcagag ctgagggagc 1681 agctgcccct gattgctggc tcggcagcgg ccggggtcgt gttcgttgtg tccttggtgg 1741 ccatctctat cgtctgtagc aggaaacggg cttatagcaa agaggctgtg tacagcgata 1801 agctccagca ttacagcaca ggccgaggct ccccagggat gaagatctac attgacccct 1861 tcacttacga ggatcccaac gaagctgtcc gggagtttgc caaggagatt gatgtatctt 1921 ttgtgaaaat tgaagaggtc atcggagcag gggagtttgg agaagtgtac aaggggcgtt 1981 tgaaactgcc aggcaagagg gaaatctacg tggccatcaa gaccctgaag gcagggtact 2041 cggagaagca gcgtcgggac tttctgagtg aggcgagcat catgggccag ttcgaccatc 2101 ctaacatcat tcgcctggag ggtgtggtca ccaagagtcg gcctgtcatg atcatcacag 2161 agttcatgga gaatggtgca ttggattctt tcctcaggca aaatgacggg cagttcaccg 2221 tgatccagct tgtgggtatg ctcaggggca tcgctgctgg catgaagtac ctggctgaga 2281 tgaattatgt gcatcgggac ctggctgcta ggaacattct ggtcaacagt aacctggtgt 2341 gcaaggtgtc cgactttggc ctctcccgct acctccagga tgacacctca gatcccacct 2401 acaccagctc cttgggaggg aagatccctg tgagatggac agctccagag gccatcgcct 2461 accgcaagtt cacttcagcc agcgacgttt ggagctatgg gatcgtcatg tgggaagtca 2521 tgtcatttgg agagagaccc tattgggata tgtccaacca aGATGTCATC AATGCCATCG 2581 AGCAGGACTA CCGGCTGCCC CCACCCATGG ACTGTCCAGC TGCTCTACAC CAGCTCATGC 2641 TGGACTGTTG GCAGAAGGAC CGGAACAGCC GGCCCCGGTT TGCGGAGATT GTCAACACCC 2701 TAGATAAGAT GATCCGGAAC CCGGCAAGTC TCAAGACTGT GGCAACCATC ACCGCCGTGC 2761 CTTCCCAGCC CCTGCTCGAC CGCTCCATCC CAGACTTCAC GGCCTTTACC ACCGTGGATG 2821 ACTGGCTCAG CGCCATCAAA ATGGTCCAGT ACAGGGACAG CTTCCTCACT GCTGGCTTCA 2881 CCTCCCTCCA GCTGGTCACC CAGATGACAT CAGAAGACCT CCTGAGAATA GGCATCACCT 2941 TGGCAGGCCA TCAGAAGAAG ATCCTGAACA GCATTCATTC TATGAGGGTC CAGATAAGTC 3001 AGTCACCAAC GGCAATGGCA TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3061 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3121 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTTT CTAGATTTTG 3181 CCGGTGTAGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt