Construct: ORF TRCN0000472501

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000110.1_s317c1
Derived from:
ccsbBroadEn_15075
DNA Barcode:
AAAAAGCCCACAAAACCTTACCCA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAP3K20 (51776)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472501
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51776 MAP3K20 mitogen-activated protein k... NM_133646.3 100% 100%
2 human 51776 MAP3K20 mitogen-activated protein k... XM_017004323.1 100% 100%
3 human 51776 MAP3K20 mitogen-activated protein k... XM_017004324.1 100% 100%
4 human 51776 MAP3K20 mitogen-activated protein k... NM_016653.3 49.7% 44.6% (many diffs)
5 human 51776 MAP3K20 mitogen-activated protein k... XM_005246640.2 49.7% 44.6% (many diffs)
6 mouse 65964 Map3k20 mitogen-activated protein k... NM_001164791.1 93.1% 96.4% (many diffs)
7 mouse 65964 Map3k20 mitogen-activated protein k... NM_178084.4 59.4% 61.9% (many diffs)
8 mouse 65964 Map3k20 mitogen-activated protein k... NM_023057.5 47.4% 43.6% (many diffs)
9 mouse 65964 Map3k20 mitogen-activated protein k... XM_006499997.2 47.4% 43.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1431
ORF length:
1365
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc gtctctcggt gcctcctttg tgcaaattaa atttgatgac ttgcagtttt
121ttgaaaactg cggtggagga agttttggga gtgtttatcg agccaaatgg atatcacagg
181acaaggaggt ggctgtaaag aagctcctca aaatagagaa agaggcagaa atactcagtg
241tcctcagtca cagaaacatc atccagtttt atggagtaat tcttgaacct cccaactatg
301gcattgtcac agaatatgct tctctgggat cactctatga ttacattaac agtaacagaa
361gtgaggagat ggatatggat cacattatga cctgggccac tgatgtagcc aaaggaatgc
421attatttaca tatggaggct cctgtcaagg tgattcacag agacctcaag tcaagaaacg
481ttgttatagc tgctgatgga gtattgaaga tctgtgactt tggtgcctct cggttccata
541accatacaac acacatgtcc ttggttggaa ctttcccatg gatggctcca gaagttatcc
601agagtctccc tgtgtcagaa acttgtgaca catattccta tggtgtggtt ctctgggaga
661tgctaacaag ggaggtcccc tttaaaggtt tggaaggatt acaagtagct tggcttgtag
721tggaaaaaaa cgagagatta accattccaa gcagttgccc cagaagtttt gctgaactgt
781tacatcagtg ttgggaagct gatgccaaga aacggccatc attcaagcaa atcatttcaa
841tcctggagtc catgtcaaat gacacgagcc ttcctgacaa gtgtaactca ttcctacaca
901acaaggcgga gtggaggtgc gaaattgagg caactcttga gaggctaaag aaactagagc
961gtgatctcag ctttaaggag caggagctta aagaacgaga aagacgttta aagatgtgGG
1021AGCAAAAGCT GACAGAGCAG TCCAACACCC CGCTTCTCTT GCCTCTTGCT GCAAGAATGT
1081CTGAGGAGTC TTACTTTGAA TCTAAAACAG AGGAGTCAAA CAGTGCAGAG ATGTCATGTC
1141AGATCACAGC AACAAGTAAC GGGGAGGGCC ATGGCATGAA CCCAAGTCTG CAGGCCATGA
1201TGCTGATGGG CTTTGGGGAT ATCTTCTCAA TGAACAAAGC AGGAGCTGTG ATGCATTCTG
1261GGATGCAGAT AAACATGCAA GCCAAGCAGA ATTCTTCCAA AACCACATCT AAGAGAAGGG
1321GGAAGAAAGT CAACATGGCT CTGGGGTTCA GTGATTTTGA CTTGTCAGAA GGTGACGATG
1381ATGATGATGA TGACGGTGAG GAGGAGGATA ATGACATGGA TAATAGTGAA TGCCCAACTT
1441TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
1501CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
1561CTTGTGGAAA GGACGAAAAA AGCCCACAAA ACCTTACCCA ACGCGTTAAG TCgacaatca
1621acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)