Construct: ORF TRCN0000472503
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018964.2_s317c1
- Derived from:
- ccsbBroadEn_14608
- DNA Barcode:
- CTAACACTTAGGATTCGTCAAAGT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DGKG (1608)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472503
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001346.3 | 99% | 27.8% | (many diffs) |
| 2 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001080744.2 | 95.9% | 28.7% | (many diffs) |
| 3 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001080745.2 | 94.1% | 29.2% | (many diffs) |
| 4 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | NM_138650.2 | 87% | 22.3% | (many diffs) |
| 5 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316850.1 | 82.2% | 21.1% | (many diffs) |
| 6 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | NM_001346673.1 | 82.2% | 23.5% | (many diffs) |
| 7 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316851.1 | 77.6% | 22.1% | (many diffs) |
| 8 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316852.1 | 72.6% | 23.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 738
- ORF length:
- 672
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg tgaagaacgg tgggtctccc tcactccaga agaatttgac caactccaga 121 aatattcaga atattcctcc aagaagataa aagatgcctt gactgaattt aatgagggtg 181 ggagcctcaa acaatatgac ccacatgagc cgattagcta tgatgtcttc aagctgttca 241 tgagggcgta cctggaggtg gaccttcccc agccactgag cactcacctc ttcctggcct 301 tcagccagaa gcccagacac gagacctctg accacccgac ggagggagcc agcaacagtg 361 aggccaacag cgcagatact aatatacaga atgcagataa tgccaccaaa gcagacgagg 421 cctgtgcccc tgatactgaa tcaaatatgg ctgagaagca agcaccagct gaagaccaag 481 tggctgcgag ccccctggaa ccccccgtcc ctcggtcttc aagctcggaa tccccagtgg 541 tgtacctgaa ggatgttgtg tgctacctgt ccctgctgga gacggggagg cctcaggata 601 agctggagtt catgtttcgc ctctatgatt cagatgagaa cggtctcctg gaccaagcgg 661 agatggattg cattgtcaac caaatgctgc atattgccca gtacctggag tgggatccca 721 cagagctgag cctatattga aggagatgct gcaagggatg gactacgacc gggacggctt 781 tgtgtctcta caggaatggg tccatggagg atgacacatc ccatgctgtc ctcctgggga 841 tgatgactct ggctccatgg ggatgggcgg cacgcctgga acatgaagca cttcaagaaa 901 ccaacctact gcaacttctg ccatatcatg ctcatgggcg tccgcaagca aggcctgtgc 961 tgcacttact gtaaatacac tgtccacgaa cgctgtgtgt ccaaaaacat tcctggttgt 1021 gtcaaaacgt actcaaaagc caaaaggagt ggtgaggtga tgcagcacgc atgggtggaa 1081 gggaactcct ccgtcaagtg tgaccggtgc cacaaaagta tcaagtgcta ccagagtgtc 1141 accgcgcggc actgcgtgtg gtgccggatg acgtttcacc gcaaatgtga attatcaacg 1201 ttgtgtgacg gtggggaact cagagaccac atcttactgc ccacctccat atgccccatc 1261 acccgggaca ggccaggtga gaagtctgat ggctgcgtgt ccgccaaggg cgaacttgtc 1321 atgcagtata agatcatccc caccccgggt acccaccccc tgctggtctt ggtgaacccc 1381 aagagtggag ggagacaagg agaaagaatt cttcggaaat tccactatct gctcaacccc 1441 aaacaagttt tcaacctgga caatgggggg cctactccag ggttgaactt tttccgtgat 1501 actccagact tccgtgtttt ggcctgtggt ggagatggga cagttggctg gattttggat 1561 tgcattgata aggccaactt tgcaaagcat ccaccagtgg ctgtcctgcc tcttggaaca 1621 ggaaatgacc ttgcccgttg tttccgcggg gaaggaggtt atgaaggggg gcagcttgac 1681 aaaaatcctg aaagacattg agcagagccc cttggtgatg ctggaccgct gcatctggna 1741 agtcatcccc agagaggaag tgaaaacggg gacccaggtc ccatacagca tcatgaacaa 1801 ctatttctcc attggtgtgg acgcttccat tgcacacaga ttccatgtga tgagagagaa 1861 acatcctgaa aaattcaaca gcaggatgaa gaacaagctg tggtactttg aatttggcac 1921 ctcggagact tttgcagcga cctgcaagaa actccacgac cacattgagt tggagtgtga 1981 tggggttggg gtggacctga gcaacatctt cctggaaggc attgccattc tcaacattcc 2041 cagcatgtac ggaggcacca atcTCTGGGG AGAAAACAAG AAGAACCGGG CTGTGATCCG 2101 GGAAAGCAGG AAGGGTGTCA CTGACCCCAA AGAACTGAAA TTCTGCGTTC AAGACCTCAG 2161 TGACCAGCTC CTTGAAGTGG TGGGGCTAGA AGGAGCCATG GAGATGGGGC AGATCTACAC 2221 CGGCCTGAAG AGTGCAGGCA GGAGGCTGGC CCAGTGCGCC TCTGTCACCA TCAGGACAAA 2281 CAAGCTGCTG CCAATGCAAG TGGATGGAGA ACCCTGGATG CAGCCATGTT GCACGATTAA 2341 AATTACTCAC AAGAACCAAG CGCCCATGAT GATGGGGCCT CCCCAGAAGA GCAGCTTCTT 2401 CTCGTTGAGA AGGAAGAGCC GTTCAAAAGA CTTGCCAACT TTCTTGTACA AAGTGGTTGA 2461 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 2521 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACTA 2581 ACACTTAGGA TTCGTCAAAG TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 2641 tgtgaaagat t