Construct: ORF TRCN0000472513
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000228.1_s317c1
- Derived from:
- ccsbBroadEn_11740
- DNA Barcode:
- TCGCCATTCCCTTTTGTTCAATTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DAAM2 (23500)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472513
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23500 | DAAM2 | dishevelled associated acti... | NM_015345.3 | 11.1% | 9.1% | (many diffs) |
| 2 | human | 23500 | DAAM2 | dishevelled associated acti... | NM_001201427.2 | 11.1% | 9.1% | (many diffs) |
| 3 | human | 23500 | DAAM2 | dishevelled associated acti... | XM_006715042.2 | 11% | 9% | (many diffs) |
| 4 | human | 23500 | DAAM2 | dishevelled associated acti... | XM_006715043.2 | 11% | 9% | (many diffs) |
| 5 | human | 23500 | DAAM2 | dishevelled associated acti... | XM_006715045.3 | 11% | 9% | (many diffs) |
| 6 | human | 23500 | DAAM2 | dishevelled associated acti... | XM_006715046.4 | 11% | 9% | (many diffs) |
| 7 | human | 23500 | DAAM2 | dishevelled associated acti... | XM_017010630.1 | 10.8% | 8.8% | (many diffs) |
| 8 | human | 23500 | DAAM2 | dishevelled associated acti... | XM_006715040.3 | 10.2% | 8.3% | (many diffs) |
| 9 | human | 23500 | DAAM2 | dishevelled associated acti... | XM_006715039.3 | 10.1% | 8.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 462
- ORF length:
- 396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cccccgcaag aggagccacc atggcctggg cttcctgtgc tgcttcgggg 121 gcagtgacat ccccgaaatc aacctccggg acaaccaccc tctgcagttc atggagttct 181 ccagccccat ctcgaacgca gaggagctca acatccgcTT TGCAGAGCTG GTGGATGAAT 241 TGGATCTCAC TGACAAAAAC CGAGAGGCTA TGTTTGCACT GCCCCCTGAG AAGAAATGGC 301 AGATCTACTG CAGCAAGAAG AAGGTGCCCT CTCTGACCCC TCTGGCCACA TCTCAGGGTT 361 CATGGCATGG GGTGGCTCTT GCTGCCTTGG CCTGCTCATG CATTCACCTG ATGTTTATTA 421 CATGCCAGCC CTGTTCTAGG TGTTGGAGGA ACAACAGTGA ATACCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGATCG CCATTCCCTT TTGTTCAATT AACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t