Construct: ORF TRCN0000472521
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010683.1_s317c1
- Derived from:
- ccsbBroadEn_03563
- DNA Barcode:
- TCGCCTAACAACGGCCTAGTCTGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCMTD2 (55251)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472521
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55251 | PCMTD2 | protein-L-isoaspartate (D-a... | NM_018257.3 | 100% | 100% | |
| 2 | human | 55251 | PCMTD2 | protein-L-isoaspartate (D-a... | NM_001104925.1 | 92.5% | 92.5% | 580_581ins81 |
| 3 | mouse | 245867 | Pcmtd2 | protein-L-isoaspartate (D-a... | NM_153594.3 | 84.3% | 90.8% | (many diffs) |
| 4 | mouse | 245867 | Pcmtd2 | protein-L-isoaspartate (D-a... | NM_001291211.1 | 65.1% | 50% | (many diffs) |
| 5 | mouse | 245867 | Pcmtd2 | protein-L-isoaspartate (D-a... | XM_006500622.3 | 64.2% | 65.4% | (many diffs) |
| 6 | mouse | 245867 | Pcmtd2 | protein-L-isoaspartate (D-a... | XM_006500626.3 | 57.9% | 63.1% | (many diffs) |
| 7 | mouse | 245867 | Pcmtd2 | protein-L-isoaspartate (D-a... | XM_006500627.3 | 57.7% | 62.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1149
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cggtgctgtg agtgctggtg aagacaatga tgagctgata gataatttga 121 aagaagcaca gtatatccgg actgagctgg tagagcaggc tttcagagct atcgatcgtg 181 cagactatta tcttgaagaa tttaaagaaa atgcttataa agacttggca tggaagcatg 241 gaaacattca cctctcagcc ccgtgcatct actcggaggt gatggaagcc ctagatctgc 301 agcctggact ctcgtttctg aacctgggca gtggcactgg gtatctcagc tccatggtgg 361 gcctcattct aggtcctttt ggtgtgaacc atggggtgga acttcactca gatgtgatag 421 agtatgcaaa gcagaaactg gacttcttca tcagaacaag tgatagtttt gacaagtttg 481 acttctgtga accttccttt gttactggga attgcctgga gatttctccg gattgttctc 541 agtatgatcg tgtatactgt ggggctggcg tgcagaaaga gcatgaagag tacatgaaga 601 atcttctcaa agtgggaggg atccttgtca tgccactgga agagaagttg actaagataa 661 cacgcacagg tccttcagct tgggaaacca aaaagattct tgctgtttct tttgctcctc 721 tgatccagcc cTGCCATTCA GAGTCAGGAA AATCAAGACT TGTCCAGTTA CCACCAGTGG 781 CAGTTCGCAG CCTCCAGGAC TTGGCTCGCA TCGCCATCCG GGGCACCATT AAAAAGATTA 841 TTCATCAGGA AACTGTGAGC AAAAACGGAA ACGGACTAAA GAACACCCCC AGGTTTAAAC 901 GAAGGAGAGT TCGCCGCCGT CGAATGGAAA CGATTGTCTT TTTGGACAAA GAAGTCTTTG 961 CCAGTCGGAT TTCCAACCCC TCAGATGACA ACAGCTGTGA AGACTTGGAA GAGGAACGGA 1021 GGGAAGAAGA AGAGAAGACC CCGCCGGAAA CAAAGCCAGA CCCCCCAGTG AACTTCCTAC 1081 GCCAGAAGGT CCTGAGCCTC CCTCTGCCAG ATCCCCTGAA ATACTACTTG CTTTATTACA 1141 GAGAAAAATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TTGTGGAAAG GACGATCGCC TAACAACGGC CTAGTCTGCA 1321 CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg tgaaagatt