Construct: ORF TRCN0000472525
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016952.1_s317c1
- Derived from:
- ccsbBroadEn_13504
- DNA Barcode:
- AGTACTGAGGTTTATGTCAATTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEDC1 (283643)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472525
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283643 | TEDC1 | tubulin epsilon and delta c... | NM_001134877.1 | 67.5% | 61.6% | (many diffs) |
| 2 | human | 283643 | TEDC1 | tubulin epsilon and delta c... | NM_001198983.1 | 67.1% | 61.3% | (many diffs) |
| 3 | human | 283643 | TEDC1 | tubulin epsilon and delta c... | NM_001134876.1 | 60.5% | 55.3% | (many diffs) |
| 4 | human | 283643 | TEDC1 | tubulin epsilon and delta c... | NM_001134875.1 | 58.2% | 58.2% | 1_378del;428_583del |
| 5 | human | 283643 | TEDC1 | tubulin epsilon and delta c... | NM_001367178.1 | 50.1% | 50.1% | 1_378del;428_583del;684_890del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 810
- ORF length:
- 744
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggcccaggcc cgagtgcctc tgggtgacga gatgactgtg tgccagatcc 121 acctgtacac acgcggctgc cacagcgacc agagccttag ccatctgtct gtcactgaag 181 cagagatgct cagggaccca gagggaggcc agcagctgct gcggactctg gagcgtgaga 241 accagcgcct ggaggctgtc ctggcgtggc ggcgctctga gctggtcttc tggcggtgga 301 tggacacggt cctgggcacc tgtgccccgg aggtgcctgc tgcagcctca cagcccacct 361 tcctgcccTG GGTCCCCGAG CGCGGGGGTG GCGAGTTGGA CCTGGTAGTG CGGGAGCTGC 421 AGGCACTGGA GGAGGAGCTG CGGGAGGCTG CGGAGCGCAG GCGGGCGGCC TGGGAGGCCA 481 AGGCTGGAGG CTGTGGACGG GGGCCAGAGT GGAGTGCCGC GCGGCGGGCC TCTCGGGAGG 541 CTGTGGAAAA GGAGCTGGGA GCTCTACAGC AGTGCTGGGA GCGAGACGGT GGCCCGGCCC 601 AGCCCCATGG GCCACACCGG CTGGTGAGAC GAGAGGATGG GGCAGCAGGG GACCGGGACC 661 TGCGGGCAGC TGTGGTGATC AGGACGCTGA GGAGCCAGGA GGCCTGCCTG GAGGCGGTGC 721 TACGTCGACT ACAGGGACAG TGTCGGCAGG AACTGGCCAG GCTGGTGGGA GCCCGCCCTG 781 GTCTCATCTG GATCCCGCCA CCTGGACGCT GCCCAACTTT CTTGTACAAA GTGGTTGATA 841 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 901 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAGTAC 961 TGAGGTTTAT GTCAATTTAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1021 tgaaagatt