Construct: ORF TRCN0000472527
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016386.1_s317c1
- Derived from:
- ccsbBroadEn_00136
- DNA Barcode:
- CCCAACTCTGTCTATTAACAGCGC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP6V1C1 (528)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472527
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 528 | ATP6V1C1 | ATPase H+ transporting V1 s... | NM_001695.5 | 99.9% | 99.7% | 1146delG |
| 2 | mouse | 66335 | Atp6v1c1 | ATPase, H+ transporting, ly... | NM_025494.3 | 89.3% | 97.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1212
- ORF length:
- 1146
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgagttctgg cttatatctg ctcctgggga gaaaacctgt cagcaaacat 121 gggagaaatt gcatgcggca acttcaaaga acaataatct tgctgtcact tccaagttca 181 atattcctga cttaaaggtt ggcacgttgg atgtcttggt tggcttgtca gatgaactgg 241 ctaaactgga tgcatttgta gaaggagtgg ttaagaaagt agctcaatac atggctgatg 301 tattggaaga tagcaaagac aaagttcaag agaatctgtt ggctaatgga gtggacttgg 361 ttacttatat aacaaggttc cagtgggaca tggccaaata tccaatcaag cagtccctga 421 aaaatatttc tgaaataatt gccaagggag taactcagat tgataatgac ctgaaatctc 481 gagcatctgc atacaataac ctgaaaggaa atcttcagaa tttggaacga aagaatgcag 541 gaagtttgct aactagaagt ctagcagaaa ttgtgaagaa ggatgacttt gttcttgatt 601 cagagtatct cgtcacatta ctggtagtag ttcccaagtt aaaccacaac gactggatta 661 agcagtatga aacactagcc gaaatggtag ttccaaggtc tagcaatgtt ctttcagagg 721 accaagacag ttacctgtgt aatgtcaccT TGTTTAGGAA GGCAGTTGAT GACTTCAGAC 781 ACAAAGCCAG AGAAAACAAA TTCATTGTTC GTGACTTCCA GTATAATGAA GAGGAGATGA 841 AAGCAGATAA AGAAGAAATG AACAGGCTTT CTACTGATAA GAAAAAACAA TTTGGACCAC 901 TTGTACGGTG GCTGAAAGTG AATTTTAGTG AAGCATTTAT TGCATGGATT CACGTGAAAG 961 CATTACGGGT TTTCGTTGAG TCTGTTTTAA GGTATGGCTT GCCAGTGAAC TTCCAAGCAA 1021 TGCTACTTCA GCCCAATAAG AAAACTTTGA AGAAACTGAG AGAAGTATTA CATGAATTGT 1081 ATAAACATCT AGACAGCAGT GCAGCAGCTA TTATTGATGC TCCTATGGAT ATTCCAGGTT 1141 TAAACCTGAG TCAACAAGAA TACTACCCCT ATGTGTACTA CAAGATTGAT TGCAACTTGC 1201 TGGAATTCAA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA 1261 CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC 1321 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACCCA ACTCTGTCTA TTAACAGCGC 1381 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt