Construct: ORF TRCN0000472542
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016141.1_s317c1
- Derived from:
- ccsbBroadEn_03820
- DNA Barcode:
- TGTCCACTACAGTGACAAGGTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALNT16 (57452)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472542
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57452 | GALNT16 | polypeptide N-acetylgalacto... | NM_001168368.2 | 100% | 100% | |
| 2 | human | 57452 | GALNT16 | polypeptide N-acetylgalacto... | NM_020692.3 | 100% | 100% | |
| 3 | human | 57452 | GALNT16 | polypeptide N-acetylgalacto... | XM_011537005.2 | 100% | 100% | |
| 4 | human | 57452 | GALNT16 | polypeptide N-acetylgalacto... | XM_011537006.3 | 100% | 100% | |
| 5 | human | 57452 | GALNT16 | polypeptide N-acetylgalacto... | XM_017021498.1 | 100% | 100% | |
| 6 | human | 57452 | GALNT16 | polypeptide N-acetylgalacto... | XM_011537007.3 | 92.1% | 92.1% | 1542_1543ins132 |
| 7 | mouse | 108760 | Galnt16 | UDP-N-acetyl-alpha-D-galact... | NM_001081421.1 | 90.2% | 96% | (many diffs) |
| 8 | mouse | 108760 | Galnt16 | UDP-N-acetyl-alpha-D-galact... | XM_006515372.3 | 71.6% | 76.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1743
- ORF length:
- 1674
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggaagatc cgcgccaatg ccatcgccat cctgaccgta gcctggatcc 121 tgggcacttt ctactactta tggcaggaca accgagccca cgcagcatcc tccggcggcc 181 ggggcgcgca gagggcaggc aggaggtcgg agcagctccg cgaggaccgc accatcccgc 241 tcattgtgac aggaactccc tcgaaaggct ttgatgagaa ggcctacctg tcggccaagc 301 agctgaaggc tggagaggac ccctacagac agcacgcctt caaccagctg gagagtgaca 361 agctgagccc agaccggccc atccgggaca cccgccatta cagctgccca tctgtgtcct 421 actcctcgga cctgccagcc accagcgtca tcatcacctt ccacaatgag gcccgttcca 481 ccctgctgcg cacagtgaag agtgtcctga accgaactcc tgccaacttg atccaggaga 541 tcattttagt ggatgacttc agctcagatc cggaagactg tctactcctg accaggatcc 601 ccaaggtcaa gtgcctgcgc aatgatcggc gggaagggct gatccggtcc cgagtgcgtg 661 gggcggacgt ggctgcagct accgttctca cctttctgga tagccactgc gaagtgaaca 721 ccgagtggct gccgcccatg ctgcagcggg tgaaggagga ccacacccgc gtggtgagtc 781 ccatcattga tgtcatcagt ctggataatt ttgcctacct tgcagcatct gctgaccttc 841 gtggagggtt cgactggagc ctgcatttca agtgggagca gatccctctt gagcagaaga 901 tgacccggac agaccccacc aggcccataa ggacgcctgt catagctgga ggaatcttcg 961 tgatcgacaa gtcctggttt aaccacttgg gaaagtatga tgcccagatg gacatctggg 1021 ggggagagaa ttttgagctc tccttcaggg tgtggatgtg tggtggcagt ctggagatcg 1081 tcccctgcag ccgggtgggc catgtcttca ggaaacggca cccctacaac ttccctgagg 1141 gtaatgccct cacctacatc aggaatacta agcgcactgc agaagtgtgg atggatgaat 1201 acaagcaata ctactatgag gcccggccct cggCCATCGG GAAGGCCTTC GGCAGTGTGG 1261 CTACGCGGAT AGAGCAGAGG AAGAAGATGA ACTGCAAGTC CTTCCGCTGG TACCTGGAGA 1321 ACGTCTACCC AGAGCTCACG GTCCCCGTGA AGGAAGCACT CCCCGGCATC ATTAAGCAGG 1381 GGGTGAACTG CTTAGAATCT CAGGGCCAGA ACACAGCTGG TGACTTCCTG CTTGGAATGG 1441 GGATCTGCAG AGGGTCTGCC AAGAACCCGC AGCCCGCCCA GGCATGGCTG TTCAGTGACC 1501 ACCTCATCCA GCAGCAGGGG AAGTGCCTGG CTGCCACCTC CACCTTAATG TCCTCCCCTG 1561 GATCCCCAGT CATACTGCAG ATGTGCAACC CTAGAGAAGG CAAGCAGAAA TGGAGGAGAA 1621 AAGGATCTTT CATCCAGCAT TCAGTCAGTG GCCTCTGCCT GGAGACAAAG CCTGCCCAGC 1681 TGGTGACCAG CAAGTGTCAG GCTGACGCCC AGGCCCAGCA GTGGCAGCTG TTGCCACACA 1741 CATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1801 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1861 GGCTTTATAT ATCTTGTGGA AAGGACGATG TCCACTACAG TGACAAGGTT TCACGCGTTA 1921 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt