Construct: ORF TRCN0000472544
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013935.1_s317c1
- Derived from:
- ccsbBroadEn_10678
- DNA Barcode:
- GTTGCCTAACTCTATTAAATGTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ANK1 (286)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472544
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 286 | ANK1 | ankyrin 1 | NM_001142445.2 | 83.9% | 83.9% | 368_442del |
| 2 | human | 286 | ANK1 | ankyrin 1 | NM_020480.5 | 83.2% | 83.2% | 301_302ins66 |
| 3 | human | 286 | ANK1 | ankyrin 1 | NM_020478.5 | 82% | 80.6% | (many diffs) |
| 4 | mouse | 11733 | Ank1 | ankyrin 1, erythroid | NM_001310436.1 | 74.6% | 75.6% | (many diffs) |
| 5 | mouse | 11733 | Ank1 | ankyrin 1, erythroid | NM_001310437.1 | 71.9% | 72.9% | (many diffs) |
| 6 | mouse | 11733 | Ank1 | ankyrin 1, erythroid | XM_006509004.2 | 62.9% | 62.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 459
- ORF length:
- 393
- Sequence:
-
1 tcTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTG GACTTTCGTC ACCCAGCTGT TGGTCACGCT GGTGCTGCTG AGCTTCTTCC 121 TGGTCAGCTG TCAGAACGTG ATGCACATTG TCAGGGGGTC CCTGTGCTTT GTGCTAAAGC 181 ACATCCACCA GGAGCTGGAC AAGGAGCTGG GGGAGAGCGA GGGCCTCAGT GACGACGAGG 241 AGACCATCTC CACCAGGGTG GTCCGGCGGC GGGTCTTCCT GAAGGGGAAT GAGTTTCAGA 301 ATATTCCAGG GGAGCAGGTG ACAGAGGAGC AATTCACGGA TGAGCAGGGC AACATTGTCA 361 CCAAGAAGAT CATTCGCAAG GTGGTTCGAC AGATAGACTT GTCCAGCGCC GATGCCGCCC 421 AGGAGCACGA GGAGGATCAC ACCTCGACAC CCAACCCCTG CTCAACTTTC TTGTACAAAG 481 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 541 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 601 ACGAGTTGCC TAACTCTATT AAATGTCTAC GCGTTAAGTC gacaatcaac ctctggatta 661 caaaatttgt gaaagatt