Transcript: Human NM_020480.5

Homo sapiens ankyrin 1 (ANK1), transcript variant 7, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
ANK1 (286)
Length:
3083
CDS:
252..581

Additional Resources:

NCBI RefSeq record:
NM_020480.5
NBCI Gene record:
ANK1 (286)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_020480.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000083718 GCCAATTTCAATACGAATCAT pLKO.1 1282 3UTR 100% 5.625 3.938 N ANK1 n/a
2 TRCN0000166364 CACACACACACACACACACAA pLKO.1 1156 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_020480.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10678 pDONR223 100% 83.2% 83.2% None 301_302ins66 n/a
2 ccsbBroad304_10678 pLX_304 0% 83.2% 83.2% V5 301_302ins66 n/a
3 TRCN0000472544 GTTGCCTAACTCTATTAAATGTCT pLX_317 100% 83.2% 83.2% V5 301_302ins66 n/a
4 ccsbBroadEn_05816 pDONR223 100% 68.4% 64.5% None (many diffs) n/a
5 ccsbBroad304_05816 pLX_304 0% 68.4% 64.5% V5 (many diffs) n/a
6 TRCN0000474255 CCGCTCAGACTCACTGCTCAAATT pLX_317 78.1% 68.4% 64.5% V5 (many diffs) n/a
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