Construct: ORF TRCN0000472548
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000174.1_s317c1
- Derived from:
- ccsbBroadEn_15674
- DNA Barcode:
- CTCAGAAGTTACCGATGAATAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE3C (9690)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472548
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9690 | UBE3C | ubiquitin protein ligase E3C | XM_005249564.4 | 38.1% | 37.9% | (many diffs) |
| 2 | human | 9690 | UBE3C | ubiquitin protein ligase E3C | NM_014671.3 | 37.2% | 37% | (many diffs) |
| 3 | human | 9690 | UBE3C | ubiquitin protein ligase E3C | XM_017012818.1 | 37.1% | 35.7% | (many diffs) |
| 4 | human | 9690 | UBE3C | ubiquitin protein ligase E3C | XR_927552.3 | 23.7% | (many diffs) | |
| 5 | mouse | 100763 | Ube3c | ubiquitin protein ligase E3C | NM_133907.3 | 31.8% | 32.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1278
- ORF length:
- 1212
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt cagcttcgaa ggcgacttca agacgcggcc caaggtgtcc cttggcggcg 121 cgagcaggaa gtattccatc caaagaagtg catttgatcg ctgtgctacc ttgtcacagt 181 ccgggggcgc ttttcccatt gctaatggcc ccaaccttac ccttttggta aggcagcttc 241 tgttttttta caaacaaaat gaagactcaa aacgtttgat atggctgtat cagaacttaa 301 ttaaacacag ctctctgttt gtcaagcagt tggatggatc tgagagactt acatgcttat 361 ttcagataaa aagattgatg agcctctgtt gcaggttgct gcaaaactgt aatgatgaca 421 gtttgaatgt tgcacttcca atgagaatgc ttgaagtatt ttcgtctgag aatacttact 481 tgcctgtttt acaagatgct agctatgtgg tgtcagtgat tgaacaaatt ttgcactaca 541 tgattcacaa tgggtattat aggtctctat atttgttgat taacagcaag cttccatcaa 601 gtattgaata ttctgattta tctcgagttc ctatagcaaa aattttgcta gagaatgttc 661 taaaaccatt gcactttact tacaactcct gtccggaagg tgcgaggcaa caagttttta 721 cagccttcac agaggagttt ctggcagcaC CTTTTACAGA TCAGATTTTT CATTTCATCA 781 TTCCGGCGCT TGCAGATGCG CAGACCGTTT TCCCTTACGA GCCCTTTCTG AATGCACTGT 841 TGTTAATAGA GAGTAGATGT TCAAGAAAGA GTGGTGGAGC ACCCTGGCTT TTCTATTTCG 901 TTTTAACTGT TGGCGAAAAT TATTTGGGGG CCCTCTCTGA GGAAGGGCTG CTGGTGTATT 961 TGCGGGTGCT GCAGACCTTC CTCTCTCAGT TACCAGTCTC TCCTGCCAGC GCGAGCTGTC 1021 ACGACTCAGC CAGTGACTCT GAGGAGGAGA GTGAAGAAGC CGACAAGCCC TCAAGCCCGG 1081 AGGATGGCAG ACTGTCAGTA TCATACATAA CAGAGGAATG CCTGAAGAAG CTGGACACAA 1141 AGCAGCAGAC CAACACCCTG CTCAACCTGG TGTGGAGGGA CTCTGCGAGC GAGGAGGTCT 1201 TCACCACCAT GGCCTCCGTC TGCCACACGC TGATGGTGCA GCACCGCATG ATGGTACCCA 1261 AAGTCAGGGT GTACAAATGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1321 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1381 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTCAGAA GTTACCGATG 1441 AATAAAAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt