Construct: ORF TRCN0000472566
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006927.1_s317c1
- Derived from:
- ccsbBroadEn_08296
- DNA Barcode:
- AATGTGCACTTTTATTGAATTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UBE2J1 (51465)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472566
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51465 | UBE2J1 | ubiquitin conjugating enzym... | NM_016021.3 | 99.8% | 99.6% | 685C>G |
| 2 | human | 51465 | UBE2J1 | ubiquitin conjugating enzym... | XM_011535887.2 | 87.3% | 87.1% | 557_558ins120;565C>G |
| 3 | human | 51465 | UBE2J1 | ubiquitin conjugating enzym... | XM_011535888.3 | 72.8% | 71% | (many diffs) |
| 4 | mouse | 56228 | Ube2j1 | ubiquitin-conjugating enzym... | NM_019586.3 | 88.3% | 92.4% | (many diffs) |
| 5 | mouse | 56228 | Ube2j1 | ubiquitin-conjugating enzym... | XM_006538107.2 | 77.2% | 81.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gacccgctac aacctgaaga gtccggctgt taaacgttta atgaaagaag 121 cggcagaatt gaaagatcca acagatcatt accatgcgca gcctttagag gataaccttt 181 ttgaatggca cttcacggtt agagggcccc cagactccga ttttgatgga ggagtttatc 241 acgggcggat agtactgcca ccagagtatc ccatgaaacc accaagcatt attctcctaa 301 cggctaatgg tcgatttgaa gtgggcaaga aaatctgttt gagcatctca ggccatcatc 361 ctgaaacttg gcagccttcg tggagtataa ggacagcatt attagccatc attgggttta 421 tgccaacaaa aggagaggga gccataggtt ctctagatta cactcctgag gaaagaagag 481 cacttgccaa aaaatcacaa gatttctgtt gtgaaggatg tggctctgcc atgaaggatg 541 tcctgttgcc tttaaaatct ggaagcgatt caagccaagc tgaccaagaa gccaaagaac 601 tggctaggca aataagcttt aaggcagaag tcaattcaTC TGGAAAGACT ATCTCTGAGT 661 CAGACTTAAA CCACTCTTTT TCACTAACTG ATTTACAAGA TGATATACCT ACAACATTCC 721 AGGGTGCTAC GGCCAGTACA TCGTACGGAG TCCAGAATTC CTCAGCAGCA TCCTTTCATC 781 AACCTACCCA ACCTGTAGCT AAGAATACCT CCATGAGCCC TCGACAGCGC CGGGCCCAGC 841 AGCAGAGTCA GAGAAGGTTG TCTACTTCAC CAGATGTAAT CCAGGGCCAC CAGCCAAGAG 901 ACAACCACAC TGATCATGGT GGGTCAGCTG TACTGATTGT CATCCTGACT TTGGCATTGG 961 CAGCTCTTAT ATTCCGACGA ATATATCTGG CAAACGAATA CATATTTGAC TTTGAGTTAT 1021 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGAAATGT GCACTTTTAT TGAATTCAAA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt