Construct: ORF TRCN0000472597
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003214.1_s317c1
- Derived from:
- ccsbBroadEn_09865
- DNA Barcode:
- TCCCGCAAGCGGTTCCATTTGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSUN6 (221078)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472597
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | NM_182543.5 | 99.8% | 99.5% | 1387T>C;1390G>A |
| 2 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | NM_001351115.2 | 99.6% | 99.3% | 229_230insAGC;1384T>C;1387G>A |
| 3 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_011519383.1 | 98.8% | 98.5% | (many diffs) |
| 4 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | NM_001351116.2 | 96.5% | 94.4% | (many diffs) |
| 5 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_024447884.1 | 96.5% | 94.4% | (many diffs) |
| 6 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_011519384.1 | 95.7% | 93.6% | (many diffs) |
| 7 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_011519385.1 | 95.7% | 93.6% | (many diffs) |
| 8 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_011519386.2 | 95.7% | 93.6% | (many diffs) |
| 9 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_017015907.2 | 95.7% | 93.6% | (many diffs) |
| 10 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | NM_001351117.2 | 88.7% | 88.4% | 73_74ins156;1231T>C;1234G>A |
| 11 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_011519387.2 | 88% | 87.7% | (many diffs) |
| 12 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_017015911.2 | 78.7% | 77.6% | (many diffs) |
| 13 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_017015910.2 | 78% | 76.9% | (many diffs) |
| 14 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XR_930471.2 | 71.6% | (many diffs) | |
| 15 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | NM_001351118.2 | 69.7% | 69.5% | 0_1ins423;964T>C;967G>A |
| 16 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_017015913.2 | 69.7% | 69.5% | 0_1ins423;964T>C;967G>A |
| 17 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_011519389.3 | 69.2% | 68.9% | (many diffs) |
| 18 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XM_017015912.2 | 69.2% | 68.9% | (many diffs) |
| 19 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XR_001747059.1 | 56.5% | (many diffs) | |
| 20 | human | 221078 | NSUN6 | NOP2/Sun RNA methyltransfer... | XR_001747060.1 | 40.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1473
- ORF length:
- 1407
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tattttccct aagatatctt tgagacctga ggttgaaaac tatcttaagg 121 aaggctttat gaataaggag attgtgactg ctttaggtaa acaagaagca gaaaggaagt 181 ttgaaacttt gttaaagcac ctgtcacatc ctccatcatt tacaactgtc agagtgaata 241 cacatttagc ctcagtacaa catgtgaaaa atctgttact tgatgaactt cagaagcagt 301 ttaatggatt aagtgttcct attcttcaac atccagacct tcaagatgtg ttacttattc 361 ctgttattgg acccagaaag aatattaaaa aacaacagtg tgaagccatt gttggagccc 421 agtgtggcaa tgcagtttta agaggagccc atgtctatgc cccaggaatt gtgtcagcat 481 cacaatttat gaaagctgga gatgttattt ctgtatactc tgatattaaa ggaaaatgta 541 agaaaggagc caaagaattt gatggaacaa aagtatttct tggaaatggg atttctgaac 601 taagccgcaa agaaatcttc agtggattac ctgaactgaa aggcatgggc ataagaatga 661 cagaaccagt atatctcagc ccttcatttg acagtgtact gccccgttac ttatttttac 721 aaaatttgcc atctgcctta gtaagtcatg tactaaatcc tcaacctgga gagaagattc 781 tagacttgtg tgcagcacct ggagggaaaa caacacacat tgcagcacta atgcatgatc 841 agggagaagt tatagcactg gataaaatct tcaacaaagt agaaaaaatc aaacagaatg 901 ccttattgtt agggctgaat tccatcaggg cattttgttt tgatggaaca aaggcggtta 961 aacttgatat ggtggaggac acagaaggag aacctccatt tctaccagaa tcctttgacc 1021 gaattcttct ggatgcaccc tgtagtggaa tgggacagag accaaacatg gcctgtactT 1081 GGTCTGTGAA GGAAGTGGCA TCATATCAGC CATTACAGCG AAAACTCTTC ACTGCAGCGG 1141 TTCAGCTGCT GAAGCCAGAG GGTGTGCTGG TTTATAGCAC GTGCACTATA ACACTGGCCG 1201 AAAATGAAGA ACAGGTTGCC TGGGCCCTGA CAAAATTTCC TTGCCTTCAG CTTCAGCCCC 1261 AGGAACCGCA GATTGGAGGA GAAGGAATGA GGGGAGCTGG GCTCTCATGT GAACAGTTGA 1321 AACAGCTGCA GCGATTTGAT CCATCGGCTG TGCCATTACC GGACACTGAC ATGGACTCTC 1381 TTAGAGAGGC CAGAAGAGAA GACATGTTGC GTCTGGCTAA TAAGGACTCT ATAGGTTTTT 1441 TTATTGCAAA ACTTATAAAA TGCAAAAGCA CATACCCAAC TTTCTTGTAC AAAGTGGTTG 1501 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1561 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 1621 CCGCAAGCGG TTCCATTTGG CCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1681 ttgtgaaaga tt