Transcript: Human XR_930471.2

PREDICTED: Homo sapiens NOP2/Sun RNA methyltransferase 6 (NSUN6), transcript variant X10, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NSUN6 (221078)
Length:
1773
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_930471.2
NBCI Gene record:
NSUN6 (221078)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_930471.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000151421 GCCTTAGTAAGTCATGTACTA pLKO.1 1106 3UTR 100% 0.495 0.693 N NSUN6 n/a
2 TRCN0000151321 GAGAAGTTATAGCACTGGATA pLKO.1 1215 3UTR 100% 4.950 3.465 N NSUN6 n/a
3 TRCN0000151575 GATGAACTTCAGAAGCAGTTT pLKO.1 641 3UTR 100% 4.950 3.465 N NSUN6 n/a
4 TRCN0000154814 GCTCTCATGTGAACAGTTGAA pLKO.1 1682 3UTR 100% 4.950 3.465 N NSUN6 n/a
5 TRCN0000151146 GCTGGAGATGTTATTTCTGTA pLKO.1 854 3UTR 100% 4.950 3.465 N NSUN6 n/a
6 TRCN0000151322 GCAAAGAAATCTTCAGTGGAT pLKO.1 966 3UTR 100% 2.640 1.848 N NSUN6 n/a
7 TRCN0000151619 GCTTTAGGTAAACAAGAAGCA pLKO.1 509 3UTR 100% 2.640 1.848 N NSUN6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_930471.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09865 pDONR223 100% 71.6% None (many diffs) n/a
2 ccsbBroad304_09865 pLX_304 0% 71.6% V5 (many diffs) n/a
3 TRCN0000472597 TCCCGCAAGCGGTTCCATTTGGCC pLX_317 28.3% 71.6% V5 (many diffs) n/a
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