Construct: ORF TRCN0000472607
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013306.1_s317c1
- Derived from:
- ccsbBroadEn_12310
- DNA Barcode:
- ACGGTGAGGGCTGTTAGTCCAACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPIRE1 (56907)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472607
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56907 | SPIRE1 | spire type actin nucleation... | NM_001128627.1 | 21% | 21% | 1_1473del |
| 2 | human | 56907 | SPIRE1 | spire type actin nucleation... | XM_024451225.1 | 20.6% | 20.6% | 1_1509del |
| 3 | human | 56907 | SPIRE1 | spire type actin nucleation... | XM_011525702.1 | 18.8% | 18.8% | 1_1689del |
| 4 | human | 56907 | SPIRE1 | spire type actin nucleation... | XM_024451224.1 | 18.8% | 18.8% | 1_1689del |
| 5 | human | 56907 | SPIRE1 | spire type actin nucleation... | NM_020148.3 | 17.6% | 17.6% | 1_1833del |
| 6 | human | 56907 | SPIRE1 | spire type actin nucleation... | NM_001128626.2 | 17.3% | 17.3% | 1_1875del |
| 7 | human | 56907 | SPIRE1 | spire type actin nucleation... | XM_005258122.4 | 16.3% | 16.3% | 1_2007del |
| 8 | human | 56907 | SPIRE1 | spire type actin nucleation... | XM_011525701.1 | 16% | 16% | 1_2049del |
| 9 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | NM_176832.2 | 22.1% | 23.8% | (many diffs) |
| 10 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | NM_194355.2 | 17.7% | 19.1% | (many diffs) |
| 11 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526207.3 | 15.6% | 16.8% | (many diffs) |
| 12 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526206.1 | 15.3% | 16.5% | (many diffs) |
| 13 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526205.1 | 15.3% | 16.5% | (many diffs) |
| 14 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526204.3 | 14.4% | 15.6% | (many diffs) |
| 15 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526203.3 | 14.2% | 15.3% | (many diffs) |
| 16 | mouse | 68166 | Spire1 | spire homolog 1 (Drosophila) | XM_006526209.3 | 14.2% | 15.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 483
- ORF length:
- 393
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCGGCTGCC CTCCAAACCA TACTCCACTC 121 TTCCTATCTT TTCATTGGGA CCTTCTGCTC TGCAAAGAGG GGAAAGTAGT ATGAGGTCAG 181 AAAAACCCTC CACTGCCCAT CATCGGCCAC TTCGGAGCAT TGCCAGGTTC TCCTCAAAAT 241 CTAAGTCTAT GGACAAATCA GATGAAGAAC TCCAGTTTCC CAAAGAGTTG ATGGAGGACT 301 GGAGCACCAT GGAGGTGTGT GTGGACTGCA AGAAGTTCAT TTCGGAAATC ATCAGTTCAA 361 GCCGGCGCAG TCTGGTGTTG GCCAACAAAA GGGCCCGATT GAAAAGGAAA ACGCAGTCTT 421 TCTACATGTC CTCGCCAGGC CCCTCGGAGT ACTGCCCTTC AGAGAGGACG ATCAGTGAGA 481 TCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 541 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 601 GGCTTTATAT ATCTTGTGGA AAGGACGAAC GGTGAGGGCT GTTAGTCCAA CGACGCGTTA 661 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt