Construct: ORF TRCN0000472609
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017679.1_s317c1
- Derived from:
- ccsbBroadEn_02386
- DNA Barcode:
- CCAACCTAATTGTATATGATGCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF1B (10289)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472609
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10289 | EIF1B | eukaryotic translation init... | NM_005875.3 | 100% | 100% | |
| 2 | human | 10209 | EIF1 | eukaryotic translation init... | NM_005801.4 | 82% | 92% | (many diffs) |
| 3 | mouse | 68969 | Eif1b | eukaryotic translation init... | NM_026892.3 | 92.3% | 100% | (many diffs) |
| 4 | mouse | 20918 | Eif1 | eukaryotic translation init... | NM_011508.2 | 81.7% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 429
- ORF length:
- 339
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCCACTAT CCAGAACCTC CAATCTTTCG 121 ACCCCTTTGC TGATGCAACT AAGGGTGACG ACTTACTCCC GGCAGGGACT GAGGATTACA 181 TTCATATAAG AATCCAGCAA CGGAACGGCA GAAAGACACT GACTACTGTT CAGGGCATTG 241 CAGATGATTA TGACAAAAAG AAACTTGTGA AAGCTTTCAA AAAGAAATTT GCCTGTAATG 301 GTACTGTGAT TGAACATCCT GAATACGGAG AGGTTATTCA GCTTCAAGGT GACCAAAGAA 361 AAAACATCTG CCAGTTTCTC TTGGAGGTTG GCATTGTAAA GGAGGAACAG CTTAAGGTTC 421 ATGGATTCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCAACC TAATTGTATA TGATGCAAAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt