Construct: ORF TRCN0000472632
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012765.1_s317c1
- Derived from:
- ccsbBroadEn_09382
- DNA Barcode:
- TTTAAACCGTCACATTTAAGTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHF21B (112885)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472632
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112885 | PHF21B | PHD finger protein 21B | NM_138415.5 | 99.9% | 99.8% | 15C>G |
| 2 | human | 112885 | PHF21B | PHD finger protein 21B | NM_001135862.3 | 92% | 91.9% | 15C>G;562_563ins126 |
| 3 | human | 112885 | PHF21B | PHD finger protein 21B | XM_006724122.1 | 92% | 91.9% | 15C>G;562_563ins126 |
| 4 | human | 112885 | PHF21B | PHD finger protein 21B | NM_001242450.1 | 89.7% | 89.2% | (many diffs) |
| 5 | human | 112885 | PHF21B | PHD finger protein 21B | NM_001284296.2 | 61.5% | 61.5% | 0_1ins486;76_77ins126 |
| 6 | human | 112885 | PHF21B | PHD finger protein 21B | XM_011529855.1 | 61.5% | 56.5% | (many diffs) |
| 7 | human | 112885 | PHF21B | PHD finger protein 21B | NR_134535.1 | 40.2% | (many diffs) | |
| 8 | mouse | 271305 | Phf21b | PHD finger protein 21B | NM_001081166.2 | 80.4% | 80.8% | (many diffs) |
| 9 | mouse | 271305 | Phf21b | PHD finger protein 21B | XM_006521034.3 | 76.4% | 76.6% | (many diffs) |
| 10 | mouse | 271305 | Phf21b | PHD finger protein 21B | XM_006521033.3 | 73.1% | 72.6% | (many diffs) |
| 11 | mouse | 271305 | Phf21b | PHD finger protein 21B | XM_017316654.1 | 53.6% | 43.6% | (many diffs) |
| 12 | mouse | 271305 | Phf21b | PHD finger protein 21B | XM_017316655.1 | 53.6% | 43.6% | (many diffs) |
| 13 | mouse | 271305 | Phf21b | PHD finger protein 21B | XM_017316656.1 | 44.8% | 43.5% | (many diffs) |
| 14 | mouse | 271305 | Phf21b | PHD finger protein 21B | NR_030731.1 | 32.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1659
- ORF length:
- 1593
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctgcagagg cggcccgagg cgctcgccgt ggaactcgcg cgccaccaga 121 acggcgacct caagaagcag ctccacgaaa ggcagccgcg gatcgccgcg ctcagcgaca 181 aacaagcttt gggaacgatc actgcagtgc ctgtcacggg tcctcaggtc agctccttgc 241 agaggttggc cgggcaagga gcggcagtgc tacctcaggt taggccaaag actctgattc 301 cagacagcct ccccgttgcc ccgggccggg accggccacc caagcagccc ccaacattcc 361 agaaggccac cgtggtcagc gtcaagaacc ccagcccagc cctccccacc gccaacaaca 421 ctgtcagcca tgtgccagcg cccggcagcc agccccaggc cctcgccgag cccgccgccc 481 tcgcctctcc gctgagcagt gcgggggtgg cctacgccat catctccacc tcccccagca 541 atgccgccgc catggccccc agcaccgccg tgtctgtggt cagtgacagc atcaaagtcc 601 agcccctcct catcagtgct gacaacaagc ctcctccacg cctcctctct tcccctcacc 661 ccgcaaccca tcactgtccc ctccacccct cctctcttcc cctcacccct ccctccccat 721 cactgtcccc ttcacccctc catggcatct tccaggtcat catcattcag cctcaagtgc 781 agacgcagcc cgagagcacg gcagagtcgc ggccgcccac agaggagcca tctcagggag 841 ctcaggccac caaaaagaag aaggaagacc ggcccccgac ccaggagaac cccgagaaaa 901 tcgccttcat ggtagcgcta ggcctggtta ccacggaaca tttggaagaa atccagagca 961 agcgacagga gcggaagaga agaagcacag ccaaccctgc ctacagcggc ctcctggaga 1021 ccgagaggaa acggctggcc tccaactatc tcaacaaccc cctgttcctc acagcgagag 1081 ccaatgagga cccctgctgg aagaacgaga tcacccacga tgagcactgt gccgcctgca 1141 agcgaggggc caacctgcag ccctgcggca cctgcccggg ggcctaccac cTCAGCTGCC 1201 TGGAGCCGCC CCTCAAGACG GCGCCCAAGG GCGTGTGGGT GTGCCCCAGG TGCCAGCAGA 1261 AGGCCTTAAA GAAAGACGAG GGTGTGCCCT GGACTGGGAT GCTGGCCATC GTGCACTCTT 1321 ATGTCACCCA CAAGACAGTC AAAGAAGAGG AGAAGCAGAA GCTGCTGCAA CGAGGCAGTG 1381 AGCTGCAGAA CGAGCACCAG CAGCTGGAGG AGCGGGACCG GCGGCTGGCG TCAGCAGTGC 1441 AGAAATGCCT GGAGTTGAAG ACAAGCCTGC TGGCCCGCCA GAGGGGCACC CAGTCATCCC 1501 TGGACCGCCT GCGGGCCCTC CTGAGACTGA TACAGGGCGA GCAGCTGCTC CAGGTCACCA 1561 TGACGACCAC TAGCCCTGCC CCACTGCTGG CCGGGCCCTG GACCAAGCCC TCAGTGGCAG 1621 CCACACACCC CACCGTCCAG CACCCCCAGG GCCACAACTG CCCAACTTTC TTGTACAAAG 1681 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1741 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1801 ACGATTTAAA CCGTCACATT TAAGTGACAC GCGTTAAGTC gacaatcaac ctctggatta 1861 caaaatttgt gaaagatt