Construct: ORF TRCN0000472636

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014252.3_s317c1
Derived from:
ccsbBroadEn_01836
DNA Barcode:
CATGCACCTCCGACAATCCGGGCT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAPKAPK3 (7867)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472636
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 7867 MAPKAPK3 MAPK activated protein kina... NM_001243925.2 100% 100%
2 human 7867 MAPKAPK3 MAPK activated protein kina... NM_001243926.1 100% 100%
3 human 7867 MAPKAPK3 MAPK activated protein kina... NM_004635.4 100% 100%
4 human 7867 MAPKAPK3 MAPK activated protein kina... XR_940501.2 40.5% 1_126del;629_630ins124;1149_2396del
5 mouse 102626 Mapkapk3 mitogen-activated protein k... NM_178907.3 89.6% 94.2% (many diffs)
6 mouse 102626 Mapkapk3 mitogen-activated protein k... XM_006511616.3 89.6% 94.2% (many diffs)
7 mouse 102626 Mapkapk3 mitogen-activated protein k... XM_006511617.3 76.4% 78.6% (many diffs)
8 mouse 102626 Mapkapk3 mitogen-activated protein k... XM_006511618.3 75% 79.1% (many diffs)
9 mouse 102626 Mapkapk3 mitogen-activated protein k... XM_006511619.1 65.7% 70.1% (many diffs)
10 mouse 102626 Mapkapk3 mitogen-activated protein k... NM_001316691.1 62.1% 58% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1212
ORF length:
1146
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga tggtgaaaca gcagaggagc aggggggccc tgtgcccccg ccagttgcac
121ccggcggacc cggcttgggc ggtgctccgg gggggcggcg ggagcccaag aagtacgcag
181tgaccgacga ctaccagttg tccaagcagg tgctgggcct gggtgtgaac ggcaaagtgc
241tggagtgctt ccatcggcgc actggacaga agtgtgccct gaagctcctg tatgacagcc
301ccaaggcccg gcaggaggta gaccatcact ggcaggcttc tggcggcccc catattgtct
361gcatcctgga tgtgtatgag aacatgcacc atggcaagcg ctgtctcctc atcatcatgg
421aatgcatgga aggtggtgag ttgttcagca ggattcagga gcgtggcgac caggctttca
481ctgagagaga agctgcagag ataatgcggg atattggcac tgccatccag tttctgcaca
541gccataacat tgcccaccga gatgtcaagc ctgaaaacct actctacaca tctaaggaga
601aagacgcagt gcttaagctc accgattttg gctttgctaa ggagaccacc caaaatgccc
661tgcagacacc ctgctatact ccctattatg tggcccctga ggtcctgggt ccagagaagt
721atgacaagtc atgtgacatg tggtccctgg gtgtcatcat gtacatcctc ctttgtggct
781tcccaccctt ctactccaac acgggccagg ccatctcccc ggggatgaag aggaggattc
841gcctgggcca gtacggcttc cccaatcctg agtggtcaga agtctctgag gatgccaagc
901agctgatccg cctcctgttg aagacagacc ccacagagag gctgaccatc actcagttca
961tgaaccaccc ctggatcaac caatcgatgg tagtgccaca gaccccactc cacaCGGCCC
1021GAGTGCTGCA GGAGGACAAA GACCACTGGG ACGAAGTCAA GGAGGAGATG ACCAGTGCCT
1081TGGCCACTAT GCGGGTAGAC TACGACCAGG TGAAGATCAA GGACCTGAAG ACCTCTAACA
1141ACCGGCTCCT CAACAAGAGG AGAAAAAAGC AGGCAGGCAG CTCCTCTGCC TCACAGGGCT
1201GCAACAACCA GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1261ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1321CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACAT GCACCTCCGA CAATCCGGGC
1381TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)