Construct: ORF TRCN0000472643
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012496.1_s317c1
- Derived from:
- ccsbBroadEn_00555
- DNA Barcode:
- ATTACATTAGCCCAGACCCTGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGF1 (2246)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472643
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_000800.5 | 100% | 100% | |
| 2 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001144892.3 | 100% | 100% | |
| 3 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001144934.2 | 100% | 100% | |
| 4 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001144935.2 | 100% | 100% | |
| 5 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001257205.1 | 100% | 100% | |
| 6 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001257207.1 | 100% | 100% | |
| 7 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001257208.2 | 100% | 100% | |
| 8 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001257209.1 | 100% | 100% | |
| 9 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001257210.1 | 100% | 100% | |
| 10 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354951.2 | 100% | 100% | |
| 11 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354952.2 | 100% | 100% | |
| 12 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354953.2 | 100% | 100% | |
| 13 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354954.2 | 100% | 100% | |
| 14 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354955.2 | 100% | 100% | |
| 15 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354956.2 | 100% | 100% | |
| 16 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001257206.2 | 99.3% | 99.3% | 273_274insCAG |
| 17 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001257211.2 | 99.3% | 99.3% | 273_274insCAG |
| 18 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001257212.2 | 99.3% | 99.3% | 273_274insCAG |
| 19 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354957.2 | 99.3% | 99.3% | 273_274insCAG |
| 20 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354958.2 | 99.3% | 99.3% | 273_274insCAG |
| 21 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354959.2 | 38.2% | 36.1% | (many diffs) |
| 22 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354961.2 | 38.2% | 36.1% | (many diffs) |
| 23 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354962.2 | 38.2% | 36.1% | (many diffs) |
| 24 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354963.2 | 38.2% | 36.1% | (many diffs) |
| 25 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_001354964.2 | 38.2% | 36.1% | (many diffs) |
| 26 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_033136.4 | 38.2% | 36.1% | (many diffs) |
| 27 | human | 2246 | FGF1 | fibroblast growth factor 1 | NM_033137.4 | 37.6% | 36.1% | (many diffs) |
| 28 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | NM_010197.3 | 90.5% | 95.4% | (many diffs) |
| 29 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_006525644.3 | 90.5% | 95.4% | (many diffs) |
| 30 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_006525645.3 | 90.5% | 95.4% | (many diffs) |
| 31 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_006525647.3 | 90.5% | 95.4% | (many diffs) |
| 32 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_006525648.3 | 90.5% | 95.4% | (many diffs) |
| 33 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_006525649.1 | 90.5% | 95.4% | (many diffs) |
| 34 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_011246842.2 | 90.5% | 95.4% | (many diffs) |
| 35 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_011246843.2 | 90.5% | 95.4% | (many diffs) |
| 36 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_017317830.1 | 90.5% | 95.4% | (many diffs) |
| 37 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_017317831.1 | 90.1% | 94.8% | (many diffs) |
| 38 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_011246841.2 | 81.5% | 86% | (many diffs) |
| 39 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_006525641.3 | 79.2% | 83.6% | (many diffs) |
| 40 | mouse | 14164 | Fgf1 | fibroblast growth factor 1 | XM_006525642.3 | 78.9% | 83% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 531
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaaggggaa atcaccacct tcacagccct gaccgagaag tttaatctgc 121 ctccagggaa ttacaagaag cccaaactcc tctactgtag caacgggggc cacttcctga 181 ggaTCCTTCC GGATGGCACA GTGGATGGGA CAAGGGACAG GAGCGACCAG CACATTCAGC 241 TGCAGCTCAG TGCGGAAAGC GTGGGGGAGG TGTATATAAA GAGTACCGAG ACTGGCCAGT 301 ACTTGGCCAT GGACACCGAC GGGCTTTTAT ACGGCTCACA GACACCAAAT GAGGAATGTT 361 TGTTCCTGGA AAGGCTGGAG GAGAACCATT ACAACACCTA TATATCCAAG AAGCATGCAG 421 AGAAGAATTG GTTTGTTGGC CTCAAGAAGA ATGGGAGCTG CAAACGCGGT CCTCGGACTC 481 ACTATGGCCA GAAAGCAATC TTGTTTCTCC CCCTGCCAGT CTCTTCTGAT TACCCAACTT 541 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 601 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 661 CTTGTGGAAA GGACGAATTA CATTAGCCCA GACCCTGCCG ACGCGTTAAG TCgacaatca 721 acctctggat tacaaaattt gtgaaagatt