Transcript: Mouse XM_006525647.3

PREDICTED: Mus musculus fibroblast growth factor 1 (Fgf1), transcript variant X10, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Fgf1 (14164)
Length:
4127
CDS:
405..872

Additional Resources:

NCBI RefSeq record:
XM_006525647.3
NBCI Gene record:
Fgf1 (14164)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006525647.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000066699 GCGAAGTGTATATAAAGGGTA pLKO.1 604 CDS 100% 2.640 3.696 N Fgf1 n/a
2 TRCN0000066701 CGCAGACACCAAATGAGGAAT pLKO.1 676 CDS 100% 4.950 3.960 N Fgf1 n/a
3 TRCN0000423024 AGGCCACAGAGCCTGAATTTG pLKO_005 948 3UTR 100% 13.200 9.240 N Fgf1 n/a
4 TRCN0000066700 CATGCGGAGAAGAACTGGTTT pLKO.1 753 CDS 100% 4.950 3.465 N Fgf1 n/a
5 TRCN0000066698 CCAGCAAATTAGAAACACTAT pLKO.1 2207 3UTR 100% 4.950 3.465 N Fgf1 n/a
6 TRCN0000066702 CCTGCCTCTAGGAAACTACAA pLKO.1 455 CDS 100% 4.950 3.465 N Fgf1 n/a
7 TRCN0000437083 TGACTAGAGGAGTCTGTTCTG pLKO_005 866 CDS 100% 4.050 2.835 N Fgf1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006525647.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00555 pDONR223 100% 90.5% 95.4% None (many diffs) n/a
2 ccsbBroad304_00555 pLX_304 0% 90.5% 95.4% V5 (many diffs) n/a
3 TRCN0000472643 ATTACATTAGCCCAGACCCTGCCG pLX_317 73.8% 90.5% 95.4% V5 (many diffs) n/a
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