Construct: ORF TRCN0000472647
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008332.1_s317c1
- Derived from:
- ccsbBroadEn_07654
- DNA Barcode:
- CCACCGCACTCGCGAAACTTGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POLR3F (10621)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472647
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10621 | POLR3F | RNA polymerase III subunit F | NM_006466.4 | 99.7% | 99.6% | 840A>G;941T>A |
| 2 | human | 10621 | POLR3F | RNA polymerase III subunit F | NM_001282526.2 | 86.8% | 86.7% | 0_1ins123;717A>G;818T>A |
| 3 | human | 10621 | POLR3F | RNA polymerase III subunit F | XM_017027596.1 | 84.5% | 84.4% | 429_430ins144;696A>G;797T>A |
| 4 | human | 10621 | POLR3F | RNA polymerase III subunit F | XM_011529141.1 | 73.2% | 73.1% | 0_1ins252;588A>G;689T>A |
| 5 | human | 10621 | POLR3F | RNA polymerase III subunit F | XR_937031.1 | 43.7% | (many diffs) | |
| 6 | human | 10621 | POLR3F | RNA polymerase III subunit F | NR_104209.2 | 42.4% | (many diffs) | |
| 7 | mouse | 70408 | Polr3f | polymerase (RNA) III (DNA d... | NM_029763.3 | 90.5% | 98.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1014
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaggtgaag gtgaaggtgc agccgcctga cgcggatccg gtcgaaatag 121 aaaacaggat tatagaatta tgtcaccagt tccctcatgg aatcacagac caagtaattc 181 agaatgaaat gcctcatata gaagcccagc agcgggcagt agccatcaat aggttgttgt 241 ctatgggtca gttggatctc ttaaggagca atacgggcct tttatataga ataaaggact 301 ctcagaatgc tggtaaaatg aagggatccg ataaccaaga aaaactagta tatcaaatca 361 tagaggatgc aggaaataaa ggaatatgga gcagagatat ccgctataaa agtaatttgc 421 cattaacaga aatcaacaaa attctgaaga atctggaaag taaaaagctt atcaaagctg 481 ttaagtctgt agcagcctca aaaaagaagg tgtatatgct ctataacctg cagccagacc 541 ggtctgtgac tggtggagcc tGGTACAGTG ACCAGGATTT TGAATCTGAA TTTGTAGAGG 601 TGCTTAACCA ACAGTGTTTT AAATTCCTAC AGTCCAAGGC AGAAACAGCA CGAGAAAGCA 661 AACAGAACCC AATGATACAA AGAAATAGTT CATTTGCCTC ATCACATGAA GTGTGGAAAT 721 ATATCTGCGA ATTGGGAATC AGTAAGGTAG AGTTATCCAT GGAAGACATT GAAACCATCC 781 TGAATACACT CATTTATGAT GGAAAAGTGG AGATGACGAT TATTGCTGCA AAAGAAGGCA 841 CAGTTGGCAG TGTAGATGGA CACATGAAAC TGTACAGGGC AGTCAATCCA ATCATCCCTC 901 CCACGGGTTT GGTCCGGGCA CCCTGTGGAC TCTGCCCGGT TTTTGATGAC TGCCACGAAG 961 GTGGTGAGAT TTCACCATCT AACTGTATTT ACATGACAGA GTGGCACGAA TTTTACCCAA 1021 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1081 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1141 TATCTTGTGG AAAGGACGAC CACCGCACTC GCGAAACTTG GGAACGCGTT AAGTCgacaa 1201 tcaacctctg gattacaaaa tttgtgaaag att