Construct: ORF TRCN0000472656
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001021.1_s317c1
- Derived from:
- ccsbBroadEn_07279
- DNA Barcode:
- TGTTTCCACATAATGTTAGATTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKR1C3 (8644)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472656
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8644 | AKR1C3 | aldo-keto reductase family ... | NM_003739.6 | 99.6% | 100% | 90G>A;312G>A;546C>T |
| 2 | human | 8644 | AKR1C3 | aldo-keto reductase family ... | NM_001253908.1 | 98.9% | 99.6% | (many diffs) |
| 3 | human | 1645 | AKR1C1 | aldo-keto reductase family ... | NM_001353.6 | 91.5% | 87.9% | (many diffs) |
| 4 | human | 1646 | AKR1C2 | aldo-keto reductase family ... | NM_001354.5 | 91.1% | 86.9% | (many diffs) |
| 5 | human | 1646 | AKR1C2 | aldo-keto reductase family ... | NM_205845.2 | 91.1% | 86.9% | (many diffs) |
| 6 | human | 1109 | AKR1C4 | aldo-keto reductase family ... | NM_001818.4 | 88.4% | 84.2% | (many diffs) |
| 7 | human | 1645 | AKR1C1 | aldo-keto reductase family ... | XM_017015791.2 | 84.5% | 82% | (many diffs) |
| 8 | human | 1646 | AKR1C2 | aldo-keto reductase family ... | NM_001321027.1 | 84.1% | 81.1% | (many diffs) |
| 9 | human | 8644 | AKR1C3 | aldo-keto reductase family ... | NM_001253909.2 | 41.2% | 39.3% | (many diffs) |
| 10 | human | 1646 | AKR1C2 | aldo-keto reductase family ... | NM_001135241.2 | 36.9% | 33.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttccaaacac cagtgtgtaa agctaaatga tggccacttc atgcctgtat 121 tgggatttgg cacctatgca cctccagagg ttccaagaag taaagctttg gaggtcacaa 181 aattagcaat agaagctggg ttccgccata tagattctgc tcatttatac aataatgagg 241 agcaggttgg actggccatc cgaagcaaga ttgcagatgg cagtgtgaag agagaagaca 301 tattctacac ttcaaagctt tggtccactt ttcatcgacc agagttggtc cgaccagcct 361 tggaaaactc actgaaaaaa gctcaattgg actatgttga cctctatctt attcattctc 421 caatgtctct aaagccaggt gaggaacttt caccaacaga tgaaaatgga aaagtaatat 481 ttgacatagt ggatctctgt accacctggg aggccatgga gaagtgtaag gatgcaggat 541 tGGCCAAGTC CATTGGGGTG TCAAACTTCA ACCGCAGGCA GCTGGAGATG ATCCTCAACA 601 AGCCAGGACT TAAGTACAAG CCTGTCTGCA ACCAGGTAGA ATGTCATCCG TATTTCAACC 661 GGAGTAAATT GCTAGATTTC TGCAAGTCGA AAGATATTGT TCTGGTTGCC TATAGTGCTC 721 TGGGATCTCA ACGAGACAAA CGATGGGTGG ACCCGAACTC CCCGGTGCTC TTGGAGGACC 781 CAGTCCTTTG TGCCTTGGCA AAAAAGCACA AGCGAACCCC AGCCCTGATT GCCCTGCGCT 841 ACCAGCTGCA GCGTGGGGTT GTGGTCCTGG CCAAGAGCTA CAATGAGCAG CGCATCAGAC 901 AGAACGTGCA GGTTTTTGAG TTCCAGTTGA CTGCAGAGGA CATGAAAGCC ATAGATGGCC 961 TAGACAGAAA TCTCCACTAT TTTAACAGTG ATAGTTTTGC TAGCCACCCT AATTATCCAT 1021 ATTCAGATGA ATATTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGTTTCCACA TAATGTTAGA 1201 TTCAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt