Construct: ORF TRCN0000472661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004147.2_s317c1
- Derived from:
- ccsbBroadEn_15014
- DNA Barcode:
- GTAGACTGAATAGGAGACAGGTTT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIK3R5 (23533)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472661
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23533 | PIK3R5 | phosphoinositide-3-kinase r... | NM_001251851.2 | 100% | 100% | |
| 2 | human | 23533 | PIK3R5 | phosphoinositide-3-kinase r... | NM_001251852.1 | 100% | 100% | |
| 3 | human | 23533 | PIK3R5 | phosphoinositide-3-kinase r... | NM_001251853.1 | 100% | 100% | |
| 4 | human | 23533 | PIK3R5 | phosphoinositide-3-kinase r... | NM_001251855.1 | 100% | 100% | |
| 5 | human | 23533 | PIK3R5 | phosphoinositide-3-kinase r... | NM_001142633.2 | 56.1% | 56.1% | 1_1158del |
| 6 | human | 23533 | PIK3R5 | phosphoinositide-3-kinase r... | NM_014308.3 | 56.1% | 56.1% | 1_1158del |
| 7 | human | 23533 | PIK3R5 | phosphoinositide-3-kinase r... | XM_005256580.3 | 56% | 56% | 1_1158del;1902_1903insCAG |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1548
- ORF length:
- 1482
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cagcggctac gtggaggaca gcgaggagag ctcctccgag tggccttgga 121 ggcgtggcag ccaggaacgc cgaggccacc gcaggcctgg gcagaagttc atcaggatct 181 ataaactctt caagagcacc agccagctgg tactgcggag ggactctcgg agcctggagg 241 gcagctcgga cacggccctg cccctgaggc gggcagggag cctctgcagc cccctggacg 301 aaccagtatc acccccttcc cgggcccagc gctcccgctc cctgccccag cccaaactcg 361 gtacccagct gcccagctgg cttctggccc ctgcttcacg cccccagcgc cgccgcccct 421 tcctgagtgg agatgaggat cccaaggctt ccacgctacg tgttgtggtc tttggctccg 481 atcggatttc agggaaggtg gctcgggcgt acagcaacct tcggcggctg gagaacaatc 541 gcccactcct cacacggttc ttcaaacttc agttcttcta cgtgcctgtg aagcgaagtc 601 atgggaccag ccctggtgcc tgtccacccc ctcggagcca gacgccctca cccccgacag 661 actcccctag gcacgccagc cctggagagc tgggcaccac cccatgggag gagagcacca 721 atgacatctc ccactacctc ggcatgctgg acccctggta tgagcgcaat gtactgggcc 781 tcatgcacct gccccctgaa gtcctgtgcc agcagtccct gaaggctgaa gcccaggccc 841 tggagggctc cccaacccag ctgcccatcc tggctgacat gctactctac tactgccgct 901 ttgccgccag accggtgctg ctgcaagtct atcagaccga gctgaccttc atcactgggg 961 agaagacgac agagatcttc atccactcct tggagctggg tcactccgct gccacacgtg 1021 ccatcaaggc gtcaggtcct ggcagcaagc ggctgggcat cgatggcgac cgggaggctg 1081 ttcctctaac actacagatt atttacagca agggggccat cagtggacga agtcgctgga 1141 gcaacctgga gaaggtctgt acctccgtga acctcaacaa ggcctgccgg AAGCAGGAGG 1201 AGCTGGATTC CAGCATGGAG GCCCTGACGC TAAACCTGAC AGAAGTGGTG AAAAGGCAGA 1261 ACTCCAAATC CAAGAAGGGC TTTAACCAGA TTAGCACATC GCAGATCAAA GTGGACAAGG 1321 TGCAGATCAT CGGCTCCAAC AGCTGCCCCT TTGCTGTGTG CCTGGACCAG GATGAGAGAA 1381 AGATCCTGCA GAGTGTAGTC AGATGTGAGG TCTCACCGTG CTACAAGCCA GAGAAGAGCG 1441 ACCTCTCCTC ACCACCCCAG ACGCCTCCTG ACCTGCCGGC CCAGGCCGCA CCTGATCTCT 1501 GCTCCCTTCT CTGCCTGCCC ATCATGACTT TCAGTGGAGC TCTGCCCTAC CCAATTTCTT 1561 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 1621 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 1681 TGGAAAGGAC GAGTAGACTG AATAGGAGAC AGGTTTACGC GTTAAGTCga caatcaacct 1741 ctggattaca aaatttgtga aagatt