Construct: ORF TRCN0000472685
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001110.1_s317c1
- Derived from:
- ccsbBroadEn_08012
- DNA Barcode:
- AGATATCTTATCCCTGGCAACTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ARFGAP3 (26286)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472685
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26286 | ARFGAP3 | ADP ribosylation factor GTP... | NM_014570.5 | 99.8% | 99.8% | 1065T>G;1353G>A;1431T>C |
| 2 | human | 26286 | ARFGAP3 | ADP ribosylation factor GTP... | XM_005261525.4 | 91.3% | 91.4% | 1062_1063ins132;1221G>A;1299T>C |
| 3 | human | 26286 | ARFGAP3 | ADP ribosylation factor GTP... | NM_001142293.2 | 91.2% | 91.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1614
- ORF length:
- 1548
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggaccccagc aagcaggaca tcttgaccat cttcaagcgc ctccgctcgg 121 tgcccactaa caaggtgtgt tttgattgtg gtgccaaaaa tcccagctgg gcaagcataa 181 cctatggagt gttcctttgc attgattgct cagggtccca ccggtcactt ggtgttcact 241 tgagttttat tcgatctaca gagttggatt ccaactggtc atggtttcag ttgcgatgca 301 tgcaagtcgg aggaaacgct agtgcatctt ccttttttca tcaacatggg tgttccacca 361 atgacaccaa tgccaagtac aacagtcgtg ctgctcagct ctatagggag aaaatcaaat 421 cgctcgcctc tcaagcaaca cggaagcatg gcactgatct gtggcttgat agttgtgtgg 481 ttccaccttt gtcccctcca ccaaaggagg aagatttttt tgcctctcac gtttctcctg 541 aggtgagtga cacagcgtgg gcatcagcaa tagcagaacc atcttcttta acatcaaggc 601 ctgtggaaac cactttggaa aataatgaag gtggacaaga gcaaggacca agtgtggaag 661 gtcttaatgt accaacaaag gctactttag aggtatcctc tatcataaaa aagaaaccaa 721 atcaagctaa aaaaggcctt ggggccaaaa aaggaagttt gggagctcag aaactggcaa 781 acacatgctt taatgaaatt gaaaaacaag ctcaagctgc ggataaaatg aaggagcagg 841 aagacctggc caaggtggta tctaaagaag aatcaattgt ttcatcatta cgattagcct 901 ataaggatct tgaaattcaa atgaagaaag acgaaaagat gaacattagt ggcaaaaaaa 961 atgttgactc agacagactc ggcatgggat ttggaaattg cagaagtgtt atttcacatt 1021 cagtgacttc agatatgcag accatagagc aggaatcacc cattatggca aaaccaagaa 1081 aaaagtataa tgatgacagt gacgattcat attttacttc cagctcaagg tactttgacg 1141 agccagtgga gttaaggagc agttctttct ctagctggga tgacagttca gattcctatt 1201 ggaaaaaaga gaccagcaaa gatactgaaa cagttctgaa aaccacaggc tattcagaca 1261 gacctactgc tcgccgcaag ccagattatg agccagttGA AAATACAGAT GAGGCCCAGA 1321 AGAAGTTTGG CAATGTCAAG GCCATTTCAT CAGATATGTA TTTTGGAAGA CAATCCCAGG 1381 CTGATTATGA GACCAGGGCC CGCCTAGAGA GGCTGTCAGC AAGTTCCTCC ATAAGCTCGG 1441 CTGATCTGTT CGAGGAGCCG AGGAAGCAGC CAGCAGGGAA CTACAGCCTG TCCAGCGTGC 1501 TGCCCAACGC CCCCGACATG GCGCAGTTCA AGCAGGGAGT GAGATCGGTT GCTGGAAAAC 1561 TCTCCGTCTT TGCTAATGGA GTCGTGACTT CAATTCAGGA TCGCTACGGT TCTTACCCAA 1621 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1681 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1741 TATCTTGTGG AAAGGACGAA GATATCTTAT CCCTGGCAAC TAAACGCGTT AAGTCgacaa 1801 tcaacctctg gattacaaaa tttgtgaaag att