Construct: ORF TRCN0000472687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006452.1_s317c1
- Derived from:
- ccsbBroadEn_12204
- DNA Barcode:
- TATCTTTATCAGCTCGATGGCCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DRAM1 (55332)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55332 | DRAM1 | DNA damage regulated autoph... | XM_017019578.1 | 73.3% | 73.3% | 1_144del |
| 2 | human | 55332 | DRAM1 | DNA damage regulated autoph... | NM_018370.3 | 55.4% | 55.4% | 1_318del |
| 3 | human | 55332 | DRAM1 | DNA damage regulated autoph... | XM_005269004.2 | 42.4% | 42.4% | 1_318del;577_578ins93 |
| 4 | mouse | 71712 | Dram1 | DNA-damage regulated autoph... | XM_011243576.1 | 63.5% | 68.3% | (many diffs) |
| 5 | mouse | 71712 | Dram1 | DNA-damage regulated autoph... | XM_017314100.1 | 63.5% | 68.3% | (many diffs) |
| 6 | mouse | 71712 | Dram1 | DNA-damage regulated autoph... | NM_027878.2 | 48% | 51.6% | (many diffs) |
| 7 | mouse | 71712 | Dram1 | DNA-damage regulated autoph... | XR_380446.3 | 25.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 462
- ORF length:
- 396
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cattGTCGCC AATTTTCAGG AGTTAGCTGT GCCAGTGGTT CATGACGGGG 121 GCGCTCTTTT GGCCTTTGTC TGTGGTGTCG TGTACACGCT CCTACAGTCC ATCATCTCTT 181 ACAAATCATG TCCCCAGTGG AACAGTCTCT CGACATGCCA CATACGGATG GTCATCTCTG 241 CCGTTTCTTG CGCAGCTGTC ATCCCCATGA TTGTCTGTGC TTCACTAATT TCCATAACCA 301 AGCTGGAGTG GAATCCAAGA GAAAAGGATT ATGTATATCA CGTAGTGAGT GCGATCTGTG 361 AATGGACAGT GGCCTTTGGT TTTATTTTCT ACTTCCTAAC TTTCATCCAA GATTTCCAGA 421 GTGTCACCCT AAGGATATCC ACAGAAATCA ATGGTGATAT TTGCCCAACT TTCTTGTACA 481 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 541 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 601 AGGACGATAT CTTTATCAGC TCGATGGCCG CACGCGTTAA GTCgacaatc aacctctgga 661 ttacaaaatt tgtgaaagat t