Construct: ORF TRCN0000472704
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004820.1_s317c1
- Derived from:
- ccsbBroadEn_12289
- DNA Barcode:
- ACTGATTACGTGAACTTCATCCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BIN3 (55909)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472704
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55909 | BIN3 | bridging integrator 3 | XM_011544586.2 | 99.8% | 100% | 432T>C |
| 2 | human | 55909 | BIN3 | bridging integrator 3 | NM_001363046.1 | 96.9% | 97% | 1_18del;450T>C |
| 3 | human | 55909 | BIN3 | bridging integrator 3 | NM_018688.6 | 78.5% | 78.6% | 1_162del;594T>C |
| 4 | human | 55909 | BIN3 | bridging integrator 3 | NR_156436.1 | 34.1% | (many diffs) | |
| 5 | human | 55909 | BIN3 | bridging integrator 3 | XM_011544587.1 | 33.2% | 26.4% | (many diffs) |
| 6 | mouse | 57784 | Bin3 | bridging integrator 3 | XM_006519331.2 | 83% | 89.4% | (many diffs) |
| 7 | mouse | 57784 | Bin3 | bridging integrator 3 | XM_006519332.3 | 83% | 89.4% | (many diffs) |
| 8 | mouse | 57784 | Bin3 | bridging integrator 3 | NM_021328.3 | 68.6% | 73.9% | (many diffs) |
| 9 | mouse | 57784 | Bin3 | bridging integrator 3 | XM_011245124.2 | 58.1% | 62.3% | (many diffs) |
| 10 | mouse | 57784 | Bin3 | bridging integrator 3 | XM_006519330.2 | 57.9% | 54.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 663
- ORF length:
- 597
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc aaaatctgcc gtgaagatat ccttggactt actctccaat cccctctgtg 121 agcaagacca ggaccttctg aacatggtga cggccctgga cacggccatg aagcggatgg 181 atgCCTTCAA TCAGGAAAAG GTGAACCAGA TCCAGAAGAC TGTGATCGAG CCCTTAAAAA 241 AGTTCGGCAG TGTCTTCCCG AGCCTCAACA TGGCTGTGAA GAGGCGGGAA CAGGCCTTGC 301 AGGACTACAG GAGGCTGCAG GCCAAGGTGG AGAAGTATGA GGAAAAGGAG AAGACGGGGC 361 CAGTGCTGGC CAAGCTCCAC CAGGCACGAG AGGAGCTGCG GCCTGTGCGG GAGGACTTTG 421 AAGCCAAGAA CAGGCAGCTG CTGGAGGAGA TGCCGCGCTT CTACGGCAGC CGCCTCGACT 481 ACTTCCAGCC CAGCTTCGAG TCCCTCATCC GAGCTCAGGT TGTGTACTAC TCGGAAATGC 541 ACAAGATCTT TGGAGACCTG TCCCATCAGC TTGACCAGCC AGGCCACTCC GATGAGCAGC 601 GGGAGCGGGA GAACGAGGCC AAACTCAGTG AGCTCCGGGC CCTCTCCATT GTGGCCGATG 661 ACTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 721 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 781 GGCTTTATAT ATCTTGTGGA AAGGACGAAC TGATTACGTG AACTTCATCC ACACGCGTTA 841 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt