Transcript: Human XM_011544586.2

PREDICTED: Homo sapiens bridging integrator 3 (BIN3), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
BIN3 (55909)
Length:
1653
CDS:
345..944

Additional Resources:

NCBI RefSeq record:
XM_011544586.2
NBCI Gene record:
BIN3 (55909)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011544586.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000275904 ATTGTGGCCGATGACTGAATC pLKO_005 927 CDS 100% 10.800 15.120 N BIN3 n/a
2 TRCN0000275905 AGCGGATGGATGCCTTCAATC pLKO_005 451 CDS 100% 10.800 8.640 N BIN3 n/a
3 TRCN0000285461 CATGCGTTGTGGGCATCAAAT pLKO_005 1285 3UTR 100% 13.200 9.240 N BIN3 n/a
4 TRCN0000275903 CGGAAATGCACAAGATCTTTG pLKO_005 811 CDS 100% 10.800 7.560 N BIN3 n/a
5 TRCN0000146413 CAGTGGAGAGAGACTTTGAAA pLKO.1 125 5UTR 100% 5.625 3.938 N BIN3 n/a
6 TRCN0000130975 CCAGATCCAGAAGACTGTGAT pLKO.1 485 CDS 100% 4.950 3.465 N BIN3 n/a
7 TRCN0000146275 CCTTTGCAATGAATGACTCTT pLKO.1 1066 3UTR 100% 4.950 3.465 N BIN3 n/a
8 TRCN0000129928 GAAGATATCCTTGGACTTACT pLKO.1 362 CDS 100% 4.950 3.465 N BIN3 n/a
9 TRCN0000275961 GAAGATATCCTTGGACTTACT pLKO_005 362 CDS 100% 4.950 3.465 N BIN3 n/a
10 TRCN0000148000 GTGAAGATATCCTTGGACTTA pLKO.1 360 CDS 100% 4.950 3.465 N BIN3 n/a
11 TRCN0000148593 CAAGAAACAGATTGTGCCCAA pLKO.1 102 5UTR 100% 2.160 1.512 N BIN3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011544586.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12289 pDONR223 100% 99.8% 100% None 432T>C n/a
2 ccsbBroad304_12289 pLX_304 0% 99.8% 100% V5 432T>C n/a
3 TRCN0000472704 ACTGATTACGTGAACTTCATCCAC pLX_317 79.4% 99.8% 100% V5 432T>C n/a
4 ccsbBroadEn_08609 pDONR223 100% 78.6% 78.6% None 0_1ins162 n/a
5 ccsbBroad304_08609 pLX_304 0% 78.6% 78.6% V5 0_1ins162 n/a
6 TRCN0000468069 CCTTACAGGTTCCCTTATCCAGAA pLX_317 1.7% 78.6% 78.6% V5 0_1ins162 n/a
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