Construct: ORF TRCN0000472723
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011799.1_s317c1
- Derived from:
- ccsbBroadEn_01535
- DNA Barcode:
- GACAGCTCCACAAACTATTGCCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST8SIA1 (6489)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472723
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6489 | ST8SIA1 | ST8 alpha-N-acetyl-neuramin... | NM_003034.3 | 100% | 100% | |
| 2 | human | 6489 | ST8SIA1 | ST8 alpha-N-acetyl-neuramin... | NM_001304450.1 | 59.8% | 55% | 0_1ins319;61_62ins110 |
| 3 | mouse | 20449 | St8sia1 | ST8 alpha-N-acetyl-neuramin... | NM_011374.2 | 87.2% | 91.5% | (many diffs) |
| 4 | mouse | 20449 | St8sia1 | ST8 alpha-N-acetyl-neuramin... | XM_011241574.1 | 69.7% | 73% | (many diffs) |
| 5 | mouse | 20449 | St8sia1 | ST8 alpha-N-acetyl-neuramin... | XM_006506928.3 | 67.7% | 72.4% | (many diffs) |
| 6 | mouse | 20449 | St8sia1 | ST8 alpha-N-acetyl-neuramin... | XM_006506929.3 | 67.7% | 72.4% | (many diffs) |
| 7 | mouse | 20449 | St8sia1 | ST8 alpha-N-acetyl-neuramin... | XM_006506930.3 | 67.7% | 72.4% | (many diffs) |
| 8 | mouse | 20449 | St8sia1 | ST8 alpha-N-acetyl-neuramin... | XM_011241575.1 | 67.7% | 72.4% | (many diffs) |
| 9 | mouse | 20449 | St8sia1 | ST8 alpha-N-acetyl-neuramin... | XM_011241576.2 | 53.2% | 50.4% | (many diffs) |
| 10 | mouse | 20449 | St8sia1 | ST8 alpha-N-acetyl-neuramin... | XM_017321472.1 | 51.1% | 49.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1137
- ORF length:
- 1068
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcccctgc gggcgggccc ggcgacaaac gtccagaggg gccatggctg 121 tactggcgtg gaagttcccg cggacccggc tgcccatggg agccagtgcc ctctgtgtcg 181 tggtcctctg ttggctctac atcttccccg tctaccggct gcccaacgag aaagagatcg 241 tgcagggggt gctgcaacag ggcacggcgt ggaggaggaa ccagaccgcg gccagagcgt 301 tcaggaaaca aatggaagac tgctgcgacc ctgcccatct ctttgctatg actaaaatga 361 attcccctat ggggaagagc atgtggtatg acggggagtt tttatactca ttcaccattg 421 acaattcaac ttactctctc ttcccacagg caaccccatt ccagctgcca ttgaagaaat 481 gcgcggtggt gggaaatggt gggattctga agaagagtgg ctgtggccgt caaatagatg 541 aagcaaattt tgtcatgcga tgcaatctcc ctcctttgtc aagtgaatac actaaggatg 601 ttggatccaa aagtcagtta gtgacagcta atcccagcat aattcggcaa aggtttcaga 661 accttctgtg gtccagaaag acatttgtgg acaacatgaa aatttataac cacagttaca 721 tctacatgcc tgccttttct atgaagacag gaacagagcc atctttgagg gtttattata 781 cactgtcaga tgttggtgcC AATCAAACAG TGCTGTTTGC CAACCCCAAC TTTCTGCGTA 841 GCATTGGAAA GTTCTGGAAA AGTAGAGGAA TCCATGCCAA GCGCCTGTCC ACAGGACTTT 901 TTCTGGTGAG CGCAGCTCTG GGTCTCTGTG AAGAGGTGGC CATCTATGGC TTCTGGCCCT 961 TCTCTGTGAA TATGCATGAG CAGCCCATCA GCCACCACTA CTATGACAAC GTCTTACCCT 1021 TTTCTGGCTT CCATGCCATG CCCGAGGAAT TTCTCCAACT CTGGTATCTT CATAAAATCG 1081 GTGCACTGAG AATGCAGCTG GACCCATGTG AAGATACCTC ACTCCAGCCC ACTTCCTTGC 1141 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GAGACAGCTC CACAAACTAT TGCCTGACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt