Construct: ORF TRCN0000472725

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002985.1_s317c1
Derived from:
ccsbBroadEn_07615
DNA Barcode:
ATCGAACCCCTACATTTCATGAGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
COG5 (10466)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472725
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10466 COG5 component of oligomeric gol... NM_001161520.2 96.1% 96.1% 0_1ins93;895T>C;1689T>G
2 human 10466 COG5 component of oligomeric gol... NM_006348.4 92% 91.9% (many diffs)
3 human 10466 COG5 component of oligomeric gol... NM_181733.3 89.6% 89.5% (many diffs)
4 human 10466 COG5 component of oligomeric gol... XM_024446634.1 69.1% 66.6% (many diffs)
5 mouse 238123 Cog5 component of oligomeric gol... XM_006515083.1 83.3% 84.7% (many diffs)
6 mouse 238123 Cog5 component of oligomeric gol... NM_001163126.1 80.6% 81.9% (many diffs)
7 mouse 238123 Cog5 component of oligomeric gol... XM_011243868.2 80.6% 81.9% (many diffs)
8 mouse 238123 Cog5 component of oligomeric gol... XM_006515084.3 75% 75.9% (many diffs)
9 mouse 238123 Cog5 component of oligomeric gol... XM_011243869.2 51.4% 52.3% (many diffs)
10 mouse 238123 Cog5 component of oligomeric gol... XM_017315052.1 51.4% 52.3% (many diffs)
11 mouse 238123 Cog5 component of oligomeric gol... XM_017315053.1 45.8% 46.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2535
ORF length:
2469
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg ctgggtgggc gggcggcgcc gggattctgc gtcaccacct gggcggagcc
121gttctgctgc tgacgacatc aacccggcac ctgccaacat ggaaggtggc ggcggcagcg
181tcgctgtagc tggcctcgga gctcgaggct ctggagcggc tgcagctaca gtccgggaac
241ttctgcagga cgggtgttat agtgactttt taaacgaaga ctttgatgta aagacttata
301cttctcaatc tattcatcaa gctgtaattg ctgaacaact agcaaaactt gcccaaggaa
361tcagtcagtt ggacagagaa ctacacttac aggttgttgc aagacatgaa gatttactgg
421cacaagcaac tgggattgag tcgttggaag gtgttcttca gatgatgcag acgagaattg
481gggctttaca gggagctgtt gataggataa aagcaaaaat tgttgaacca tacaataaga
541tagttgcccg gactgcacaa ctagcaagac ttcaggttgc ctgtgatttg cttcggagga
601ttattcgtat cttgaatctc agtaagagac tccaaggaca actgcaaggg ggaagtagag
661agataacaaa agctgctcag agtctcaatg aacttgatta tctttctcaa ggaatagatc
721tttctggaat agaagtgata gaaaatgatc tactttttat tgcaagagcc cgacttgaag
781tggaaaatca agctaagcgc ctactagagc agggtttgga gactcagaat ccaactcaag
841tcggaacagc tcttcaggtt ttctataatc ttggaacttt gaaggatact attaccagtg
901ttgtggatgg atattgtgct actttagaag aaaatatcaa cagtgcatta gacataaaag
961ttttgactca gccttcccag tcagctgtga gagggggacc tggacgatct accatgccaa
1021ccccaggaaa tactgcagct ttgcgtgcct cactctggac caatatggag aaacttatgg
1081atcatattta tgctgtttgt ggacaggtac aacatctaca aaaagtattg gccaagaaga
1141gagatcctgt ttctcacatt tgtttcattg aagaaatagt taaggatgga caaccggaaa
1201ttttctacac attttggaat tcagttactc aggcactttc ttctcaattt catatggcaa
1261caaactcttc gatgtttttg aagcaggcat ttgaaggaga ataccctaaa ttattacgtc
1321tttataatga cttatggaag cgtcttcaac aatacagtca gcatatccaa gggaatttta
1381atgcaagtgg aactacagac ctctatgttg acctacaaca catggaagat gatgcacaag
1441atatattcat accaaaaaag ccagattatg atccagaaaa ggctttgaaa gactcactac
1501aaccctatga ggctgcttat ctatcaaaat ccttatctcg actcttcgat cctatcaact
1561tggtttttcc cccgggtggt cgtaatcctc cttcctctga tgaacttgat ggtattatta
1621aaactatagc aagtgaacta aatgttgctg ctgttgatac aaacctcaca ttagctgtgt
1681caaaaaatgt ggcaaagacc atccagttat acagtgtaaa atcagagcag cttctctcca
1741cacaaggaga tgcaagtcag gtgattgggc ctcttactga aggacagaga agaaatgtgg
1801cagtagtgaa ttcattgtat aagttgcacc aatcagtaac aaaggtggtt tccagtcaga
1861gctcattccc actggcagct gagcaaacta taatttcagc tctaaaggct attcatgctc
1921ttatggaaaa tgctgtgcaa cccttactca cttctgtggg agatgctata gaggccataa
1981tcatcaccat gcatcaagaa gacttttctg ggtcattatc cagctcagga aaacctgatg
2041ttccttgttc tctgtacatg aaggagctac aaggtttcat tgccagagtt atgagtgact
2101attttaaaCA CTTTGAATGC TTGGATTTTG TCTTTGACAA CACTGAGGCT ATTGCCCAAA
2161GAGCTGTTGA ACTTTTTATC CGCCATGCCA GTCTCATAAG ACCTCTTGGT GAAGGTGGGA
2221AAATGCGACT TGCTGCTGAT TTTGCACAGA TGGAGTTGGC TGTGGGTCCA TTCTGTAGAC
2281GAGTATCTGA TTTAGGAAAG TCCTATCGGA TGCTGAGATC ATTCAGACCT CTGCTCTTCC
2341AGGCAAGTGA ACATGTAGCC AGTAGTCCTG CATTGGGGGA TGTGATTCCG TTCAGCATCA
2401TTATTCAGTT TTTGTTCACG AGAGCACCCG CTGAACTGAA ATCTCCTTTC CAGAGGGCAG
2461AGTGGTCCCA CACACGCTTC TCTCAGTGGC TGGATGACCA TCCATCTGAA AAGGACAGGC
2521TCCTCCTCAT CAGGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
2581CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
2641TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATCGAACCCC TACATTTCAT
2701GAGGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)