Construct: ORF TRCN0000472725
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002985.1_s317c1
- Derived from:
- ccsbBroadEn_07615
- DNA Barcode:
- ATCGAACCCCTACATTTCATGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COG5 (10466)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472725
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 10466 | COG5 | component of oligomeric gol... | NM_001161520.2 | 96.1% | 96.1% | 0_1ins93;895T>C;1689T>G |
2 | human | 10466 | COG5 | component of oligomeric gol... | NM_006348.4 | 92% | 91.9% | (many diffs) |
3 | human | 10466 | COG5 | component of oligomeric gol... | NM_181733.3 | 89.6% | 89.5% | (many diffs) |
4 | human | 10466 | COG5 | component of oligomeric gol... | XM_024446634.1 | 69.1% | 66.6% | (many diffs) |
5 | mouse | 238123 | Cog5 | component of oligomeric gol... | XM_006515083.1 | 83.3% | 84.7% | (many diffs) |
6 | mouse | 238123 | Cog5 | component of oligomeric gol... | NM_001163126.1 | 80.6% | 81.9% | (many diffs) |
7 | mouse | 238123 | Cog5 | component of oligomeric gol... | XM_011243868.2 | 80.6% | 81.9% | (many diffs) |
8 | mouse | 238123 | Cog5 | component of oligomeric gol... | XM_006515084.3 | 75% | 75.9% | (many diffs) |
9 | mouse | 238123 | Cog5 | component of oligomeric gol... | XM_011243869.2 | 51.4% | 52.3% | (many diffs) |
10 | mouse | 238123 | Cog5 | component of oligomeric gol... | XM_017315052.1 | 51.4% | 52.3% | (many diffs) |
11 | mouse | 238123 | Cog5 | component of oligomeric gol... | XM_017315053.1 | 45.8% | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2535
- ORF length:
- 2469
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgggtgggc gggcggcgcc gggattctgc gtcaccacct gggcggagcc 121 gttctgctgc tgacgacatc aacccggcac ctgccaacat ggaaggtggc ggcggcagcg 181 tcgctgtagc tggcctcgga gctcgaggct ctggagcggc tgcagctaca gtccgggaac 241 ttctgcagga cgggtgttat agtgactttt taaacgaaga ctttgatgta aagacttata 301 cttctcaatc tattcatcaa gctgtaattg ctgaacaact agcaaaactt gcccaaggaa 361 tcagtcagtt ggacagagaa ctacacttac aggttgttgc aagacatgaa gatttactgg 421 cacaagcaac tgggattgag tcgttggaag gtgttcttca gatgatgcag acgagaattg 481 gggctttaca gggagctgtt gataggataa aagcaaaaat tgttgaacca tacaataaga 541 tagttgcccg gactgcacaa ctagcaagac ttcaggttgc ctgtgatttg cttcggagga 601 ttattcgtat cttgaatctc agtaagagac tccaaggaca actgcaaggg ggaagtagag 661 agataacaaa agctgctcag agtctcaatg aacttgatta tctttctcaa ggaatagatc 721 tttctggaat agaagtgata gaaaatgatc tactttttat tgcaagagcc cgacttgaag 781 tggaaaatca agctaagcgc ctactagagc agggtttgga gactcagaat ccaactcaag 841 tcggaacagc tcttcaggtt ttctataatc ttggaacttt gaaggatact attaccagtg 901 ttgtggatgg atattgtgct actttagaag aaaatatcaa cagtgcatta gacataaaag 961 ttttgactca gccttcccag tcagctgtga gagggggacc tggacgatct accatgccaa 1021 ccccaggaaa tactgcagct ttgcgtgcct cactctggac caatatggag aaacttatgg 1081 atcatattta tgctgtttgt ggacaggtac aacatctaca aaaagtattg gccaagaaga 1141 gagatcctgt ttctcacatt tgtttcattg aagaaatagt taaggatgga caaccggaaa 1201 ttttctacac attttggaat tcagttactc aggcactttc ttctcaattt catatggcaa 1261 caaactcttc gatgtttttg aagcaggcat ttgaaggaga ataccctaaa ttattacgtc 1321 tttataatga cttatggaag cgtcttcaac aatacagtca gcatatccaa gggaatttta 1381 atgcaagtgg aactacagac ctctatgttg acctacaaca catggaagat gatgcacaag 1441 atatattcat accaaaaaag ccagattatg atccagaaaa ggctttgaaa gactcactac 1501 aaccctatga ggctgcttat ctatcaaaat ccttatctcg actcttcgat cctatcaact 1561 tggtttttcc cccgggtggt cgtaatcctc cttcctctga tgaacttgat ggtattatta 1621 aaactatagc aagtgaacta aatgttgctg ctgttgatac aaacctcaca ttagctgtgt 1681 caaaaaatgt ggcaaagacc atccagttat acagtgtaaa atcagagcag cttctctcca 1741 cacaaggaga tgcaagtcag gtgattgggc ctcttactga aggacagaga agaaatgtgg 1801 cagtagtgaa ttcattgtat aagttgcacc aatcagtaac aaaggtggtt tccagtcaga 1861 gctcattccc actggcagct gagcaaacta taatttcagc tctaaaggct attcatgctc 1921 ttatggaaaa tgctgtgcaa cccttactca cttctgtggg agatgctata gaggccataa 1981 tcatcaccat gcatcaagaa gacttttctg ggtcattatc cagctcagga aaacctgatg 2041 ttccttgttc tctgtacatg aaggagctac aaggtttcat tgccagagtt atgagtgact 2101 attttaaaCA CTTTGAATGC TTGGATTTTG TCTTTGACAA CACTGAGGCT ATTGCCCAAA 2161 GAGCTGTTGA ACTTTTTATC CGCCATGCCA GTCTCATAAG ACCTCTTGGT GAAGGTGGGA 2221 AAATGCGACT TGCTGCTGAT TTTGCACAGA TGGAGTTGGC TGTGGGTCCA TTCTGTAGAC 2281 GAGTATCTGA TTTAGGAAAG TCCTATCGGA TGCTGAGATC ATTCAGACCT CTGCTCTTCC 2341 AGGCAAGTGA ACATGTAGCC AGTAGTCCTG CATTGGGGGA TGTGATTCCG TTCAGCATCA 2401 TTATTCAGTT TTTGTTCACG AGAGCACCCG CTGAACTGAA ATCTCCTTTC CAGAGGGCAG 2461 AGTGGTCCCA CACACGCTTC TCTCAGTGGC TGGATGACCA TCCATCTGAA AAGGACAGGC 2521 TCCTCCTCAT CAGGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2581 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2641 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATCGAACCCC TACATTTCAT 2701 GAGGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt