Construct: ORF TRCN0000472729
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001315.1_s317c1
- Derived from:
- ccsbBroadEn_08154
- DNA Barcode:
- TACGAATTGCCGAAGCTCGATTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SCHIP1 (29970)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472729
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 29970 | SCHIP1 | schwannomin interacting pro... | NM_001197109.1 | 99.8% | 99.5% | 713C>T |
| 2 | human | 29970 | SCHIP1 | schwannomin interacting pro... | NM_001197108.1 | 93.9% | 90.7% | (many diffs) |
| 3 | human | 29970 | SCHIP1 | schwannomin interacting pro... | NM_001197107.1 | 50.7% | 49.1% | (many diffs) |
| 4 | human | 29970 | SCHIP1 | schwannomin interacting pro... | NM_014575.3 | 49% | 48.2% | (many diffs) |
| 5 | human | 100505385 | IQCJ-SCHIP1 | IQCJ-SCHIP1 readthrough | NM_001197114.1 | 44.6% | 43.8% | (many diffs) |
| 6 | human | 100505385 | IQCJ-SCHIP1 | IQCJ-SCHIP1 readthrough | NM_001197113.1 | 42.4% | 41.7% | (many diffs) |
| 7 | mouse | 30953 | Schip1 | schwannomin interacting pro... | NM_013928.5 | 91.9% | 98.7% | (many diffs) |
| 8 | mouse | 30953 | Schip1 | schwannomin interacting pro... | NM_001113420.1 | 85.4% | 89.6% | (many diffs) |
| 9 | mouse | 30953 | Schip1 | schwannomin interacting pro... | NM_001282044.1 | 48.3% | 51.3% | (many diffs) |
| 10 | mouse | 30953 | Schip1 | schwannomin interacting pro... | NM_001282045.1 | 48.3% | 51.3% | (many diffs) |
| 11 | mouse | 30953 | Schip1 | schwannomin interacting pro... | NM_001113421.1 | 45.6% | 48.3% | (many diffs) |
| 12 | mouse | 100505386 | Gm21949 | predicted gene, 21949 | NM_001113419.2 | 39.5% | 41.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 798
- ORF length:
- 732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt acatcaggat aactgctcgt atcaggcaca gaaaaatgag agagagtcta 121 tcagacagaa gttggcactt ggaagcttct ttgatgatgg cccaggaatt tataccagct 181 gtagcaaaag tgggaagcca agcctttcct cccgactgca gagtgggatg aacttgcaga 241 tatgctttgt caacgacagt ggcagtgata aggacagtga tgctgatgac agtaagactg 301 aaaccagctt ggacaccccc ttgtctccca tgagcaaaca gagttcttcc tattctgata 361 gagacactac tgaagaggag tcTGAATCCT TGGATGACAT GGACTTCCTT ACAAGGCAAA 421 AGAAATTGCA AGCTGAAGCC AAAATGGCCC TTGCCATGGC CAAACCAATG GCCAAAATGC 481 AAGTAGAAGT GGAGAAACAG AACAGGAAAA AGTCTCCCGT CGCTGATCTT CTGCCACACA 541 TGCCTCATAT AAGTGAATGC TTGATGAAAA GAAGTTTAAA ACCCACCGAC CTGAGAGACA 601 TGACTATTGG GCAGCTACAA GTGATAGTCA ATGATCTCCA TTCCCAGATA GAAAGCTTGA 661 ATGAAGAGTT GGTCCAGCTG CTTCTCATCC GAGATGAGCT GCACACAGAG CAGGATGCCA 721 TGCTGGTGGA CATTGAAGAC TTGACCAGAC ATGCTGAAAG TCAGCAGAAG CACATGGTAG 781 AGAAAATGCC TGCAAAGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 841 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 901 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATACGAAT TGCCGAAGCT 961 CGATTACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt