Construct: ORF TRCN0000472729

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001315.1_s317c1
Derived from:
ccsbBroadEn_08154
DNA Barcode:
TACGAATTGCCGAAGCTCGATTAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SCHIP1 (29970)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472729
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 29970 SCHIP1 schwannomin interacting pro... NM_001197109.1 99.8% 99.5% 713C>T
2 human 29970 SCHIP1 schwannomin interacting pro... NM_001197108.1 93.9% 90.7% (many diffs)
3 human 29970 SCHIP1 schwannomin interacting pro... NM_001197107.1 50.7% 49.1% (many diffs)
4 human 29970 SCHIP1 schwannomin interacting pro... NM_014575.3 49% 48.2% (many diffs)
5 human 100505385 IQCJ-SCHIP1 IQCJ-SCHIP1 readthrough NM_001197114.1 44.6% 43.8% (many diffs)
6 human 100505385 IQCJ-SCHIP1 IQCJ-SCHIP1 readthrough NM_001197113.1 42.4% 41.7% (many diffs)
7 mouse 30953 Schip1 schwannomin interacting pro... NM_013928.5 91.9% 98.7% (many diffs)
8 mouse 30953 Schip1 schwannomin interacting pro... NM_001113420.1 85.4% 89.6% (many diffs)
9 mouse 30953 Schip1 schwannomin interacting pro... NM_001282044.1 48.3% 51.3% (many diffs)
10 mouse 30953 Schip1 schwannomin interacting pro... NM_001282045.1 48.3% 51.3% (many diffs)
11 mouse 30953 Schip1 schwannomin interacting pro... NM_001113421.1 45.6% 48.3% (many diffs)
12 mouse 100505386 Gm21949 predicted gene, 21949 NM_001113419.2 39.5% 41.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
798
ORF length:
732
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt acatcaggat aactgctcgt atcaggcaca gaaaaatgag agagagtcta
121tcagacagaa gttggcactt ggaagcttct ttgatgatgg cccaggaatt tataccagct
181gtagcaaaag tgggaagcca agcctttcct cccgactgca gagtgggatg aacttgcaga
241tatgctttgt caacgacagt ggcagtgata aggacagtga tgctgatgac agtaagactg
301aaaccagctt ggacaccccc ttgtctccca tgagcaaaca gagttcttcc tattctgata
361gagacactac tgaagaggag tcTGAATCCT TGGATGACAT GGACTTCCTT ACAAGGCAAA
421AGAAATTGCA AGCTGAAGCC AAAATGGCCC TTGCCATGGC CAAACCAATG GCCAAAATGC
481AAGTAGAAGT GGAGAAACAG AACAGGAAAA AGTCTCCCGT CGCTGATCTT CTGCCACACA
541TGCCTCATAT AAGTGAATGC TTGATGAAAA GAAGTTTAAA ACCCACCGAC CTGAGAGACA
601TGACTATTGG GCAGCTACAA GTGATAGTCA ATGATCTCCA TTCCCAGATA GAAAGCTTGA
661ATGAAGAGTT GGTCCAGCTG CTTCTCATCC GAGATGAGCT GCACACAGAG CAGGATGCCA
721TGCTGGTGGA CATTGAAGAC TTGACCAGAC ATGCTGAAAG TCAGCAGAAG CACATGGTAG
781AGAAAATGCC TGCAAAGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
841TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
901GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATACGAAT TGCCGAAGCT
961CGATTACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)