Construct: ORF TRCN0000472743
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017272.1_s317c1
- Derived from:
- ccsbBroadEn_02205
- DNA Barcode:
- TCTCTAGTGGTGCGCTGTTTATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WTAP (9589)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472743
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9589 | WTAP | WT1 associated protein | NM_152857.2 | 100% | 100% | |
| 2 | human | 9589 | WTAP | WT1 associated protein | NM_152858.2 | 100% | 100% | |
| 3 | human | 9589 | WTAP | WT1 associated protein | NM_001270532.1 | 88.6% | 88.8% | 453_510delinsG |
| 4 | human | 9589 | WTAP | WT1 associated protein | NM_001270533.2 | 88.6% | 88.8% | 453_510delinsG |
| 5 | human | 9589 | WTAP | WT1 associated protein | XM_017011516.2 | 80.5% | 80.7% | 1_51del;504_561delinsG |
| 6 | human | 9589 | WTAP | WT1 associated protein | NM_001270531.2 | 38% | 37.8% | 453_1188delinsG |
| 7 | human | 9589 | WTAP | WT1 associated protein | NM_004906.5 | 38% | 37.8% | 453_1188delinsG |
| 8 | human | 9589 | WTAP | WT1 associated protein | XM_024446597.1 | 38% | 37.8% | 453_1188delinsG |
| 9 | human | 9589 | WTAP | WT1 associated protein | XM_017011514.2 | 36.4% | 36.3% | 1_51del;504_1239delinsG |
| 10 | human | 9589 | WTAP | WT1 associated protein | XM_017011515.1 | 31.2% | 30.8% | 0_1ins70;3_4insAGTTAATTCTA;372_1107delinsG |
| 11 | mouse | 60532 | Wtap | Wilms tumour 1-associating ... | NM_001113532.1 | 93.3% | 100% | (many diffs) |
| 12 | mouse | 60532 | Wtap | Wilms tumour 1-associating ... | NM_175394.2 | 93.3% | 100% | (many diffs) |
| 13 | mouse | 60532 | Wtap | Wilms tumour 1-associating ... | NM_001113533.1 | 35.5% | 37.8% | (many diffs) |
| 14 | mouse | 60532 | Wtap | Wilms tumour 1-associating ... | XM_006523341.3 | 35.5% | 37.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 519
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg taCAAAAAAG 61 TTGGCATGAC CAACGAAGAA CCTCTTCCCA AGAAGGTTCG ATTGAGTGAA ACAGACTTCA 121 AAGTTATGGC AAGAGATGAG TTAATTCTAA GATGGAAACA ATATGAAGCA TATGTACAAG 181 CTTTGGAGGG CAAGTACACA GATCTTAACT CTAATGATGT AACTGGCCTA AGAGAGTCTG 241 AAGAAAAACT AAAGCAACAA CAGCAGGAGT CTGCACGCAG GGAAAACATC CTTGTAATGC 301 GACTAGCAAC CAAGGAACAA GAGATGCAAG AGTGTACTAC TCAAATCCAG TACCTCAAGC 361 AAGTCCAGCA GCCGAGCGTT GCCCAACTGA GATCAACAAT GGTAGACCCA GCGATCAACT 421 TGTTTTTCCT AAAAATGAAA GGTGAACTGG AACAGACTAA AGACAAACTG GAACAAGCCC 481 AAAATGAACT GAGTGCCTGG AAGTTTACGC CTGATAGGTA CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGATCTCTA GTGGTGCGCT GTTTATACAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt