Construct: ORF TRCN0000472748
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004765.1_s317c1
- Derived from:
- ccsbBroadEn_11963
- DNA Barcode:
- ATTCAGGCAAATGCCAGCTAATAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUSAP1 (51203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472748
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_017022295.1 | 91.4% | 91.4% | 1_60del;74_76delAGC |
| 2 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_005254430.5 | 52.9% | 52.9% | 1_600del |
| 3 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_001243143.2 | 52.8% | 52.8% | 1_600del;614_616delAGC |
| 4 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_006720561.3 | 52% | 52% | 1_618del;632_634delAGC |
| 5 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_001243142.2 | 51.2% | 51.2% | 1_642del |
| 6 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_001301136.2 | 51.1% | 51.1% | 1_642del;656_658delAGC |
| 7 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_018454.8 | 51.1% | 51.1% | 1_645del |
| 8 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_016359.5 | 51% | 51% | 1_645del;659_661delAGC |
| 9 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_005254428.3 | 50.4% | 50.4% | 1_663del |
| 10 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_006720560.4 | 50.4% | 50.4% | 1_660del;674_676delAGC |
| 11 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_006720559.3 | 50.3% | 50.3% | 1_663del;677_679delAGC |
| 12 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | NM_001243144.2 | 44.6% | 44.6% | 1_576del;933_934ins117 |
| 13 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_017022294.2 | 43.7% | 43.7% | 1_600del;957_958ins117 |
| 14 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_006720563.3 | 42.2% | 42.2% | 1_645del;1002_1003ins117 |
| 15 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_005254431.3 | 41.7% | 41.7% | 1_663del;1020_1021ins117 |
| 16 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XM_006720562.3 | 41.6% | 41.6% | 1_663del;677_679delAGC;1023_1024ins117 |
| 17 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XR_001751300.2 | 26.9% | (many diffs) | |
| 18 | human | 51203 | NUSAP1 | nucleolar and spindle assoc... | XR_001751299.2 | 26.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 741
- ORF length:
- 675
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tgaactgaag cagcccatca ataagggagg ggtcaggact ccagtacctc 121 caagaggaag actctctgtg gcttctactc ccatcagcca acgacgctcg caaggccggt 181 cttgtggccc tgcaagtcag agtaccttgg gtctgaaggg gtcactcaag cgcTCTGCTA 241 TCTCTGCAGC TAAAACGGGT GTCAGGTTTT CAGCTGCTAC TAAAGATAAT GAGCATAAGC 301 GTTCACTGAC CAAGACTCCA GCCAGAAAGT CTGCACATGT GACCGTGTCT GGGGGCACCC 361 CAAAAGGCGA GGCTGTGCTT GGGACACACA AATTAAAGAC CATCACGGGG AATTCTGCTG 421 CTGTTATTAC CCCATTCAAG TTGACAACTG AGGCAACGCA GACTCCAGTC TCCAATAAGA 481 AACCAGTGTT TGATCTTAAA GCAAGTTTGT CTCGTCCCCT CAACTATGAA CCACACAAAG 541 GAAAGCTAAA ACCATGGGGG CAATCTAAAG AAAATAATTA TCTAAATCAA CATGTCAACA 601 GAATTAACTT CTACAAGAAA ACTTACAAAC AACCCCATCT CCAGACAAAG GAAGAGCAAC 661 GGAAGAAACG CGAGCAAGAA CGAAAGGAGA AGAAAGCAAA GGTTTTGGGA ATGCGAAGGG 721 GCCTCATTTT GGCTGAAGAT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 781 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 841 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAATTC AGGCAAATGC 901 CAGCTAATAA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt