Construct: ORF TRCN0000472771
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017735.1_s317c1
- Derived from:
- ccsbBroadEn_08994
- DNA Barcode:
- ATTAAATATTACGGAAAGGTGGGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SYNDIG1 (79953)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472771
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79953 | SYNDIG1 | synapse differentiation ind... | NM_001323606.1 | 99.8% | 100% | 339C>A |
| 2 | human | 79953 | SYNDIG1 | synapse differentiation ind... | NM_001323607.1 | 99.8% | 100% | 339C>A |
| 3 | human | 79953 | SYNDIG1 | synapse differentiation ind... | NM_024893.3 | 99.8% | 100% | 339C>A |
| 4 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_017028065.1 | 99.8% | 100% | 339C>A |
| 5 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_017028066.2 | 99.8% | 100% | 339C>A |
| 6 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_017028067.2 | 99.8% | 100% | 339C>A |
| 7 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_017028068.1 | 99.8% | 100% | 339C>A |
| 8 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_011529356.3 | 83.6% | 79.7% | (many diffs) |
| 9 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_011529347.3 | 82.9% | 77.5% | (many diffs) |
| 10 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_011529348.3 | 82.9% | 77.5% | (many diffs) |
| 11 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_011529349.3 | 82.9% | 77.5% | (many diffs) |
| 12 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_011529350.1 | 82.9% | 77.5% | (many diffs) |
| 13 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_011529351.1 | 82.9% | 77.5% | (many diffs) |
| 14 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_011529352.2 | 82.9% | 77.5% | (many diffs) |
| 15 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_011529353.2 | 82.9% | 77.5% | (many diffs) |
| 16 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_011529354.2 | 82.9% | 77.5% | (many diffs) |
| 17 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_017028064.1 | 82.9% | 77.5% | (many diffs) |
| 18 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_011529358.3 | 79.7% | 79.8% | 339C>A;618_619ins156 |
| 19 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XM_017028069.2 | 61.8% | 62% | 339C>A;480_481ins294 |
| 20 | human | 79953 | SYNDIG1 | synapse differentiation ind... | XR_937144.3 | 28.7% | (many diffs) | |
| 21 | human | 79953 | SYNDIG1 | synapse differentiation ind... | NR_147606.1 | 26.9% | (many diffs) | |
| 22 | mouse | 433485 | Syndig1 | synapse differentiation ind... | NM_001085521.2 | 87.7% | 93% | (many diffs) |
| 23 | mouse | 433485 | Syndig1 | synapse differentiation ind... | XM_006499874.3 | 87.7% | 93% | (many diffs) |
| 24 | mouse | 433485 | Syndig1 | synapse differentiation ind... | XM_011239685.2 | 87.7% | 93% | (many diffs) |
| 25 | mouse | 433485 | Syndig1 | synapse differentiation ind... | XM_011239687.2 | 87.7% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 840
- ORF length:
- 774
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tggcatcatt gaacagaaga gcatgctggt gcacagtaaa atcagtgatg 121 ctggcaagag gaatggttta attaacacca gaaacttgat ggccgagagc agagatggtc 181 tggtgtctgt ttacccagcg ccccagtacc agagccaccg ggtgggggcc agcacagtgc 241 cggccagcct ggacagcagc aggagtgagc cgatgcagca gctgctggac cccaacaccc 301 tgcagcagtc agtggagtcc cgctaccggc ccaacatcaT CCTCTATTCA GAGGGCGTGC 361 TGCGCTCCTG GGGGGACGGT GTGGCCGCCG ACTGCTGCGA GACAACCTTC ATCGAGGACC 421 GGTCGCCCAC CAAAGACAGC CTCGAGTACC CGGATGGGAA GTTCATTGAC CTCTCAGCTG 481 ATGACATAAA AATCCACACC CTGTCCTACG ATGTGGAGGA GGAGGAGGAG TTCCAGGAGC 541 TGGAGAGCGA CTACTCAAGC GACACAGAGA GTGAGGACAA TTTCCTCATG ATGCCCCCGC 601 GGGACCACCT GGGCCTCAGT GTCTTCTCCA TGCTCTGCTG CTTCTGGCCT CTGGGCATCG 661 CAGCCTTCTA CTTGTCCCAT GAGACCAACA AAGCCGTGGC CAAGGGGGAC TTGCACCAGG 721 CCAGCACCAG CTCCCGGCGG GCCCTATTCC TGGCAGTGCT GTCCATCACC ATTGGGACTG 781 GCGTCTATGT GGGCGTGGCC GTGGCCCTCA TCGCCTACCT CTCCAAGAAC AACCACCTGT 841 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 901 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 961 TTTATATATC TTGTGGAAAG GACGAATTAA ATATTACGGA AAGGTGGGAA CGCGTTAAGT 1021 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt