Construct: ORF TRCN0000472772
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008558.1_s317c1
- Derived from:
- ccsbBroadEn_13983
- DNA Barcode:
- CCTCGGTCAGCGATTTATAACCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- YWHAE (7531)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472772
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7531 | YWHAE | tyrosine 3-monooxygenase/tr... | NM_006761.5 | 99.7% | 1.5% | 10delC;764A>C |
| 2 | human | 7531 | YWHAE | tyrosine 3-monooxygenase/tr... | XM_005256784.4 | 93% | 1.6% | 10delC;716A>G;718_720delTCCins48 |
| 3 | human | 7531 | YWHAE | tyrosine 3-monooxygenase/tr... | XM_017025005.2 | 84.7% | 4% | 0_1ins65;650A>G;652_654delTCCins48 |
| 4 | human | 7531 | YWHAE | tyrosine 3-monooxygenase/tr... | NR_024058.1 | 41% | (many diffs) | |
| 5 | human | 649395 | YWHAEP1 | tyrosine 3-monooxygenase/tr... | NR_029404.1 | 36.9% | (many diffs) | |
| 6 | mouse | 22627 | Ywhae | tyrosine 3-monooxygenase/tr... | NM_009536.4 | 93.2% | 1.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 144
- ORF length:
- 78
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgatgagagg atctggtgta ccaggcgaag ctggccgagc aggctgagcg 121 atacgacgaa atggtggagt caatgaagaa agtagcaggg atggatgtgg agctgacagt 181 tgaagaaaga aacctcctat ctgttgcata taagaatgtg attggagcta gaagagcctc 241 ctggagaata atcagcagca ttGAACAGAA AGAAGAAAAC AAGGGAGGAG AAGACAAGCT 301 AAAAATGATT CGGGAATATC GGCAAATGGT TGAGACTGAG CTAAAGTTAA TCTGTTGTGA 361 CATTCTGGAT GTACTGGACA AACACCTCAT TCCAGCAGCT AACACTGGCG AGTCCAAGGT 421 TTTCTATTAT AAAATGAAAG GGGACTACCA CAGGTATCTG GCAGAATTTG CCACAGGAAA 481 CGACAGGAAG GAGGCTGCGG AGAACAGCCT AGTGGCTTAT AAAGCTGCTA GTGATATTGC 541 AATGACAGAA CTTCCACCAA CGCATCCTAT TCGCTTAGGT CTTGCTCTCA ATTTTTCCGT 601 ATTCTACTAC GAAATTCTTA ATTCCCCTGA CCGTGCCTGC AGGTTGGCAA AAGCAGCTTT 661 TGATGATGCA ATTGCAGAAC TGGATACGCT GAGTGAAGAA AGCTATAAGG ACTCTACACT 721 TATCATGCAG TTGTTACGTG ATAATCTGAC ACTATGGACT TCAGACATGC AGGGTGACGG 781 TGAAGAGCAG AATAAAGAAG CGCTGCAGGA CGTGGAAGAC GAAAATCCGT GCCCAACTTT 841 CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC 901 TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 961 TTGTGGAAAG GACGACCTCG GTCAGCGATT TATAACCTTA CGCGTTAAGT Cgacaatcaa 1021 cctctggatt acaaaatttg tgaaagatt