Construct: ORF TRCN0000472781
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006469.1_s317c1
- Derived from:
- ccsbBroadEn_15797
- DNA Barcode:
- ATGCAAACTCCAACCCTCAAAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EML4 (27436)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472781
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27436 | EML4 | EMAP like 4 | XR_001738709.1 | 3.3% | (many diffs) | |
| 2 | human | 27436 | EML4 | EMAP like 4 | XR_001738708.1 | 3.3% | (many diffs) | |
| 3 | human | 27436 | EML4 | EMAP like 4 | XR_001738710.1 | 3.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 221
- ORF end:
- 407
- ORF length:
- 186
- Sequence:
-
1 GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA AGTTAGGCCA 61 GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG ATCTTGGTTC 121 ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC 181 TAGCATCGAT TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGGGCTATA CTCCCTGCAA 241 GAAATATACT GACATGAACA GGCAGTTCTT GGAGAAGAAA GAGCATTTCT TTAAGTACCT 301 GGGGAATACA GCTCTCAGTG ATCAGCAGGG AGTTTATTTG AGGACATCAG TCACCTTTGG 361 GGTTGCCATG TACAATGAGA TTTATAATCC TGATACTCTT CGGTGGTACC CAACTTTCTT 421 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 481 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 541 TGGAAAGGAC GAATGCAAAC TCCAACCCTC AAAGTCACGC GTTAAGTCga caatcaacct 601 ctggattaca aaatttgtga aagatt