Construct: ORF TRCN0000472783
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019040.2_s317c1
- Derived from:
- ccsbBroadEn_14623
- DNA Barcode:
- ACTTTAAATTTAGCCATGCGTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EPHA2 (1969)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472783
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1969 | EPHA2 | EPH receptor A2 | NM_004431.5 | 100% | 100% | |
| 2 | human | 1969 | EPHA2 | EPH receptor A2 | NM_001329090.2 | 94.4% | 94.4% | 0_1ins162 |
| 3 | human | 1969 | EPHA2 | EPH receptor A2 | XM_017000537.1 | 58.3% | 57.5% | (many diffs) |
| 4 | mouse | 13836 | Epha2 | Eph receptor A2 | NM_010139.3 | 86.6% | 92.7% | (many diffs) |
| 5 | mouse | 13836 | Epha2 | Eph receptor A2 | XM_006538528.3 | 62.6% | 67.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2997
- ORF length:
- 2928
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagctccag gcagcccgcg cctgcttcgc cctgctgtgg ggctgtgcgc 121 tggccgcggc cgcggcggcg cagggcaagg aagtggtact gctggacttt gctgcagctg 181 gaggggagct cggctggctc acacacccgt atggcaaagg gtgggacctg atgcagaaca 241 tcatgaatga catgccgatc tacatgtact ccgtgtgcaa cgtgatgtct ggcgaccagg 301 acaactggct ccgcaccaac tgggtgtacc gaggagaggc tgagcgtatc ttcattgagc 361 tcaagtttac tgtacgtgac tgcaacagct tccctggtgg cgccagctcc tgcaaggaga 421 ctttcaacct ctactatgcc gagtcggacc tggactacgg caccaacttc cagaagcgcc 481 tgttcaccaa gattgacacc attgcgcccg atgagatcac cgtcagcagc gacttcgagg 541 cacgccacgt gaagctgaac gtggaggagc gctccgtggg gccgctcacc cgcaaaggct 601 tctacctggc cttccaggat atcggtgcct gtgtggcgct gctctccgtc cgtgtctact 661 acaagaagtg ccccgagctg ctgcagggcc tggcccactt ccctgagacc atcgccggct 721 ctgatgcacc ttccctggcc actgtggccg gcacctgtgt ggaccatgcc gtggtgccac 781 cggggggtga agagccccgt atgcactgtg cagtggatgg cgagtggctg gtgcccattg 841 ggcagtgcct gtgccaggca ggctacgaga aggtggagga tgcctgccag gcctgctcgc 901 ctggattttt taagtttgag gcatctgaga gcccctgctt ggagtgccct gagcacacgc 961 tgccatcccc tgagggtgcc acctcctgcg agtgtgagga aggcttcttc cgggcacctc 1021 aggacccagc gtcgatgcct tgcacacgac ccccctccgc cccacactac ctcacagccg 1081 tgggcatggg tgccaaggtg gagctgcgct ggacgccccc tcaggacagc gggggccgcg 1141 aggacattgt ctacagcgtc acctgcgaac agtgctggcc cgagtctggg gaatgcgggc 1201 cgtgtgaggc cagtgtgcgc tactcggagc ctcctcacgg actgacccgc accagtgtga 1261 cagtgagcga cctggagccc cacatgaact acaccttcac cgtggaggcc cgcaatggcg 1321 tctcaggcct ggtaaccagc cgcagcttcc gtactgccag tgtcagcatc aaccagacag 1381 agccccccaa ggtgaggctg gagggccgca gcaccacctc gcttagcgtc tcctggagca 1441 tccccccgcc gcagcagagc cgagtgtgga agtacgaggt cacttaccgc aagaagggag 1501 actccaacag ctacaatgtg cgccgcaccg agggtttctc cgtgaccctg gacgacctgg 1561 ccccagacac cacctacctg gtccaggtgc aggcactgac gcaggagggc cagggggccg 1621 gcagcaaggt gcacgaattc cagacgctgt ccccggaggg atctggcaac ttggcggtga 1681 ttggcggcgt ggctgtcggt gtggtcctgc ttctggtgct ggcaggagtt ggcttcttta 1741 tccaccgcag gaggaagaac cagcgtgccc gccagtcccc ggaggacgtt tacttctcca 1801 agtcagaaca actgaagccc ctgaagacat acgtggaccc ccacacatat gaggacccca 1861 accaggctgt gttgaagttc actaccgaga tccatccatc ctgtgtcact cggcagaagg 1921 tgatcggagc aggagagttt ggggaggtgt acaagggcat gctgaagaca tcctcgggga 1981 agaaggaggt gccggtggcc atcaagacgc tgaaagccgg ctacacagag aagcagcgag 2041 tggacttcct cggcgaggcc ggcatcatgg gccagttcag ccaccacaac atcatccgcc 2101 tagagggcgt catctccaaa tacaagccca tgatgatcat cactgagtac atggagaatg 2161 gggccctgga caagttcctt cgggagaagg atggcgagtt cagcgtgctg cagctggtgg 2221 gcatgctgcg gggcatcgca gctggcatga agtacctggc caacatgaac tatgtgcacc 2281 gtgacctggc tgcccgcaac atcctcgtca acagcaacct ggtctgcaag gtgtctgact 2341 ttggcctgtc ccgcgtgctg gaggacgacc ccgaggccac ctacaccacc agtggcggca 2401 agatccccat ccgctggacc gccccggagg ccatttccta ccggaagttc acctctgcca 2461 gcgacgtgtg gagctttggc attgtcatgt gggaggtgat gacctatggc gagcggccct 2521 actgggagtt gtccaaccac gaggtgatga aagccatcaa tgatggcttc cggctcccca 2581 cacccatgga ctgccccTCC GCCATCTACC AGCTCATGAT GCAGTGCTGG CAGCAGGAGC 2641 GTGCCCGCCG CCCCAAGTTC GCTGACATCG TCAGCATCCT GGACAAGCTC ATTCGTGCCC 2701 CTGACTCCCT CAAGACCCTG GCTGACTTTG ACCCCCGCGT GTCTATCCGG CTCCCCAGCA 2761 CGAGCGGCTC GGAGGGGGTG CCCTTCCGCA CGGTGTCCGA GTGGCTGGAG TCCATCAAGA 2821 TGCAGCAGTA TACGGAGCAC TTCATGGCGG CCGGCTACAC TGCCATCGAG AAGGTGGTGC 2881 AGATGACCAA CGACGACATC AAGAGGATTG GGGTGCGGCT GCCCGGCCAC CAGAAGCGCA 2941 TCGCCTACAG CCTGCTGGGA CTCAAGGACC AGGTGAACAC TGTGGGGATC CCCATCTTGC 3001 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 3061 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 3121 ATATATCTTG TGGAAAGGAC GAACTTTAAA TTTAGCCATG CGTCATACGC GTTAAGTCga 3181 caatcaacct ctggattaca aaatttgtga aagatt