Construct: ORF TRCN0000472783

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019040.2_s317c1
Derived from:
ccsbBroadEn_14623
DNA Barcode:
ACTTTAAATTTAGCCATGCGTCAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EPHA2 (1969)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472783
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1969 EPHA2 EPH receptor A2 NM_004431.5 100% 100%
2 human 1969 EPHA2 EPH receptor A2 NM_001329090.2 94.4% 94.4% 0_1ins162
3 human 1969 EPHA2 EPH receptor A2 XM_017000537.1 58.3% 57.5% (many diffs)
4 mouse 13836 Epha2 Eph receptor A2 NM_010139.3 86.6% 92.7% (many diffs)
5 mouse 13836 Epha2 Eph receptor A2 XM_006538528.3 62.6% 67.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2997
ORF length:
2928
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggagctccag gcagcccgcg cctgcttcgc cctgctgtgg ggctgtgcgc
121tggccgcggc cgcggcggcg cagggcaagg aagtggtact gctggacttt gctgcagctg
181gaggggagct cggctggctc acacacccgt atggcaaagg gtgggacctg atgcagaaca
241tcatgaatga catgccgatc tacatgtact ccgtgtgcaa cgtgatgtct ggcgaccagg
301acaactggct ccgcaccaac tgggtgtacc gaggagaggc tgagcgtatc ttcattgagc
361tcaagtttac tgtacgtgac tgcaacagct tccctggtgg cgccagctcc tgcaaggaga
421ctttcaacct ctactatgcc gagtcggacc tggactacgg caccaacttc cagaagcgcc
481tgttcaccaa gattgacacc attgcgcccg atgagatcac cgtcagcagc gacttcgagg
541cacgccacgt gaagctgaac gtggaggagc gctccgtggg gccgctcacc cgcaaaggct
601tctacctggc cttccaggat atcggtgcct gtgtggcgct gctctccgtc cgtgtctact
661acaagaagtg ccccgagctg ctgcagggcc tggcccactt ccctgagacc atcgccggct
721ctgatgcacc ttccctggcc actgtggccg gcacctgtgt ggaccatgcc gtggtgccac
781cggggggtga agagccccgt atgcactgtg cagtggatgg cgagtggctg gtgcccattg
841ggcagtgcct gtgccaggca ggctacgaga aggtggagga tgcctgccag gcctgctcgc
901ctggattttt taagtttgag gcatctgaga gcccctgctt ggagtgccct gagcacacgc
961tgccatcccc tgagggtgcc acctcctgcg agtgtgagga aggcttcttc cgggcacctc
1021aggacccagc gtcgatgcct tgcacacgac ccccctccgc cccacactac ctcacagccg
1081tgggcatggg tgccaaggtg gagctgcgct ggacgccccc tcaggacagc gggggccgcg
1141aggacattgt ctacagcgtc acctgcgaac agtgctggcc cgagtctggg gaatgcgggc
1201cgtgtgaggc cagtgtgcgc tactcggagc ctcctcacgg actgacccgc accagtgtga
1261cagtgagcga cctggagccc cacatgaact acaccttcac cgtggaggcc cgcaatggcg
1321tctcaggcct ggtaaccagc cgcagcttcc gtactgccag tgtcagcatc aaccagacag
1381agccccccaa ggtgaggctg gagggccgca gcaccacctc gcttagcgtc tcctggagca
1441tccccccgcc gcagcagagc cgagtgtgga agtacgaggt cacttaccgc aagaagggag
1501actccaacag ctacaatgtg cgccgcaccg agggtttctc cgtgaccctg gacgacctgg
1561ccccagacac cacctacctg gtccaggtgc aggcactgac gcaggagggc cagggggccg
1621gcagcaaggt gcacgaattc cagacgctgt ccccggaggg atctggcaac ttggcggtga
1681ttggcggcgt ggctgtcggt gtggtcctgc ttctggtgct ggcaggagtt ggcttcttta
1741tccaccgcag gaggaagaac cagcgtgccc gccagtcccc ggaggacgtt tacttctcca
1801agtcagaaca actgaagccc ctgaagacat acgtggaccc ccacacatat gaggacccca
1861accaggctgt gttgaagttc actaccgaga tccatccatc ctgtgtcact cggcagaagg
1921tgatcggagc aggagagttt ggggaggtgt acaagggcat gctgaagaca tcctcgggga
1981agaaggaggt gccggtggcc atcaagacgc tgaaagccgg ctacacagag aagcagcgag
2041tggacttcct cggcgaggcc ggcatcatgg gccagttcag ccaccacaac atcatccgcc
2101tagagggcgt catctccaaa tacaagccca tgatgatcat cactgagtac atggagaatg
2161gggccctgga caagttcctt cgggagaagg atggcgagtt cagcgtgctg cagctggtgg
2221gcatgctgcg gggcatcgca gctggcatga agtacctggc caacatgaac tatgtgcacc
2281gtgacctggc tgcccgcaac atcctcgtca acagcaacct ggtctgcaag gtgtctgact
2341ttggcctgtc ccgcgtgctg gaggacgacc ccgaggccac ctacaccacc agtggcggca
2401agatccccat ccgctggacc gccccggagg ccatttccta ccggaagttc acctctgcca
2461gcgacgtgtg gagctttggc attgtcatgt gggaggtgat gacctatggc gagcggccct
2521actgggagtt gtccaaccac gaggtgatga aagccatcaa tgatggcttc cggctcccca
2581cacccatgga ctgccccTCC GCCATCTACC AGCTCATGAT GCAGTGCTGG CAGCAGGAGC
2641GTGCCCGCCG CCCCAAGTTC GCTGACATCG TCAGCATCCT GGACAAGCTC ATTCGTGCCC
2701CTGACTCCCT CAAGACCCTG GCTGACTTTG ACCCCCGCGT GTCTATCCGG CTCCCCAGCA
2761CGAGCGGCTC GGAGGGGGTG CCCTTCCGCA CGGTGTCCGA GTGGCTGGAG TCCATCAAGA
2821TGCAGCAGTA TACGGAGCAC TTCATGGCGG CCGGCTACAC TGCCATCGAG AAGGTGGTGC
2881AGATGACCAA CGACGACATC AAGAGGATTG GGGTGCGGCT GCCCGGCCAC CAGAAGCGCA
2941TCGCCTACAG CCTGCTGGGA CTCAAGGACC AGGTGAACAC TGTGGGGATC CCCATCTTGC
3001CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
3061TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
3121ATATATCTTG TGGAAAGGAC GAACTTTAAA TTTAGCCATG CGTCATACGC GTTAAGTCga
3181caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)