Transcript: Mouse XM_006538528.3

PREDICTED: Mus musculus Eph receptor A2 (Epha2), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Epha2 (13836)
Length:
3214
CDS:
237..2348

Additional Resources:

NCBI RefSeq record:
XM_006538528.3
NBCI Gene record:
Epha2 (13836)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006538528.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000321951 CGATATCGACGAGGGACATTT pLKO_005 2624 3UTR 100% 13.200 18.480 N Epha2 n/a
2 TRCN0000023656 GCCAGTTTAGCCACCACAATA pLKO.1 1420 CDS 100% 13.200 10.560 N Epha2 n/a
3 TRCN0000321877 GCCAGTTTAGCCACCACAATA pLKO_005 1420 CDS 100% 13.200 10.560 N Epha2 n/a
4 TRCN0000321879 GAGTTTGGAGAGGTCTATAAA pLKO_005 1284 CDS 100% 15.000 10.500 N Epha2 n/a
5 TRCN0000023655 CCAGCGTCAGTATTAACCAAA pLKO.1 706 CDS 100% 4.950 3.465 N Epha2 n/a
6 TRCN0000023658 CGAGGTCATGAAAGCCATCAA pLKO.1 1889 CDS 100% 4.950 3.465 N Epha2 n/a
7 TRCN0000321878 CGAGGTCATGAAAGCCATCAA pLKO_005 1889 CDS 100% 4.950 3.465 N Epha2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006538528.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14623 pDONR223 0% 62.6% 67.6% None (many diffs) n/a
2 ccsbBroad304_14623 pLX_304 0% 62.6% 67.6% V5 (many diffs) n/a
3 TRCN0000472783 ACTTTAAATTTAGCCATGCGTCAT pLX_317 13.8% 62.6% 67.6% V5 (many diffs) n/a
4 TRCN0000491615 ATGCTCTTGGTCATTGGCCTATCC pLX_317 13.9% 62.6% 67.5% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000489900 AAAATATTGGTATGATACTCCATA pLX_317 13.8% 62.6% 67.4% V5 (many diffs) n/a
6 TRCN0000491591 ACATCAATAAGTGTTTTATAATAT pLX_317 26.6% 47.6% .5% V5 (not translated due to prior stop codon) (many diffs) n/a
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