Construct: ORF TRCN0000472789

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010348.3_s317c1
Derived from:
ccsbBroadEn_15123
DNA Barcode:
TATCAATTTGCTAGCACAGCTGGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SCYL1 (57410)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472789
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 57410 SCYL1 SCY1 like pseudokinase 1 XM_017018033.1 61.3% 56.9% (many diffs)
2 human 57410 SCYL1 SCY1 like pseudokinase 1 XM_024448620.1 60.3% 56.1% (many diffs)
3 human 57410 SCYL1 SCY1 like pseudokinase 1 XM_005274120.4 54.4% 54.4% 1_1068del
4 human 57410 SCYL1 SCY1 like pseudokinase 1 XM_005274121.4 54.2% 54.2% 1_1068del;1957_1958insAGC
5 human 57410 SCYL1 SCY1 like pseudokinase 1 NM_020680.4 52.5% 49% 1_1068del;2248_2302del;2399_2424del
6 human 57410 SCYL1 SCY1 like pseudokinase 1 XM_005274118.4 52.4% 48.8% (many diffs)
7 human 57410 SCYL1 SCY1 like pseudokinase 1 XM_017018031.2 52.2% 52.2% 1_1068del;1814_1815ins51
8 human 57410 SCYL1 SCY1 like pseudokinase 1 NM_001048218.2 50.4% 46.9% (many diffs)
9 human 57410 SCYL1 SCY1 like pseudokinase 1 XM_017018030.2 50.3% 46.8% (many diffs)
10 human 57410 SCYL1 SCY1 like pseudokinase 1 XM_024448619.1 50.1% 46.7% (many diffs)
11 mouse 78891 Scyl1 SCY1-like 1 (S. cerevisiae) NM_023912.2 44.5% 43.2% (many diffs)
12 mouse 78891 Scyl1 SCY1-like 1 (S. cerevisiae) XM_006531856.2 44.4% 43% (many diffs)
13 mouse 78891 Scyl1 SCY1-like 1 (S. cerevisiae) XM_006531857.2 43.1% 41.9% (many diffs)
14 mouse 78891 Scyl1 SCY1-like 1 (S. cerevisiae) XR_388273.2 22.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1341
ORF length:
1275
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt ctcatccact gaccgggcca tgcgcatccg cctcctgcag cagatggagc
121agttcatcca gtaccttgac gagccaacag tcaacaccca gatcttcccc cacgtcgtac
181atggcttcct ggacaccaac cctgccatcc gggagcagac ggtcaagtcc atgctgctcc
241tggccccaaa gctgaacgag gccaacctca atgtggagct gatgaagcac tttgcacggc
301tacaggccaa ggatgaacag ggccccatcc gctgcaacac cacagtctgc ctgggcaaaa
361tcggctccta cctcagtgct agcaccagac acagggtcct tacctctgcc ttcagccgag
421ccactaggga cccgtttgca ccgtcccggg ttgcgggtgt cctgggcttt gctgccaccc
481acaacctcta ctcaatgaac gactgtgccc agaagatcct gcctgtgctc tgcggtctca
541ctgtagatcc tgagaaatcc gtgcgagacc aggccttcaa ggccattcgg agcttcctgt
601ccaaattgga gtctgtgtcg gaggacccga cccagctgga ggaagtggag aaggatgtcc
661atgcagcctc cagccctggc atgggaggag ccgcagctag ctgggcaggc tgggccgtga
721ccggggtctc ctcactcacc tccaagctga tccgttcgca cccaaccact gccccaacag
781aaaccaacat tccccaaaga cccacgcctg aaggagttcc tgccccagcc cccacccctg
841ttcctgccac ccctacaacc tcaggccact gggagacgca ggaggaggac aaggacacag
901cagaggacag cagcactgct gacagatggg acgacgaaga ctggggcagc cTGGAGCAGG
961AGGCCGAGTC TGTGCTGGCC CAGCAGGACG ACTGGAGCAC CGGGGGCCAA GTGAGCCGTG
1021CTAGTCAGGT CAGCAACTCC GACCACAAAT CCTCCAAATC CCCAGAGTCC GACTGGAGCA
1081GCTGGGAAGC TGAGGGCTCC TGGGAACAGG GCTGGCAGGA GCCAAGCTCC CAGGAGCCAC
1141CTCCTGACGG TACACGGCTG GCCAGCGAGT ATAACTGGGG TGGCCCAGAG TCCAGCGACA
1201AGGGCGACCC CTTCGCTACC CTGTCTGCAC GTCCCAGCAC CCAGGACAGG TCAAGGCTGA
1261GCTGGCCCGG AAGAAGCGCG AGGAGCGGCG GCGGGAGATG GAGGCCAAAC GCGCCGAGAG
1321GAAGGTGGCC AAGGGCCCCA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
1381CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
1441AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATATC AATTTGCTAG
1501CACAGCTGGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)