Construct: ORF TRCN0000472800

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001511.1_s317c1
Derived from:
ccsbBroadEn_06493
DNA Barcode:
CCAGACGATTGTAAGTCAATAGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KLKB1 (3818)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472800
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3818 KLKB1 kallikrein B1 NM_000892.5 99.9% 99.8% 428G>A
2 human 3818 KLKB1 kallikrein B1 XM_011531930.2 98.2% 98.1% 428G>A;597_629del
3 human 3818 KLKB1 kallikrein B1 XM_017008181.1 98.2% 98.1% 428G>A;597_629del
4 human 3818 KLKB1 kallikrein B1 XM_017008183.1 86.4% 82.5% 428G>A;1582_1583ins140;1656_1657ins118
5 human 3818 KLKB1 kallikrein B1 XM_017008182.1 85% 81.1% (many diffs)
6 human 3818 KLKB1 kallikrein B1 NM_001318394.2 80.5% 76.5% (many diffs)
7 human 3818 KLKB1 kallikrein B1 NM_001318396.2 65.6% 61.8% 0_1ins637;114_144del
8 human 3818 KLKB1 kallikrein B1 XM_017008184.1 65.6% 61.8% 0_1ins637;114_144del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1983
ORF length:
1914
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gattttattc aagcaagcaa cttatttcat ttccttgttt gctacagttt
121cctgtggatg tctgactcaa ctctatgaaa acgccttctt cagaggtggg gatgtagctt
181ccatgtacac cccaaatgcc caatactgcc agatgaggtg cacattccac ccaaggtgtt
241tgctattcag ttttcttcca gcaagttcaa tcaatgacat ggagaaaagg tttggttgct
301tcttgaaaga tagtgttaca ggaaccctgc caaaagtaca tcgaacaggt gcagtttctg
361gacattcctt gaagcaatgt ggtcatcaaa taagtgcttg ccatcgagac atttataaag
421gagttgatat gagaggagtc aattttaatg tgtctaaggt tagcagtgtt gaagaatgcc
481aaaaaaggtg caccaataac attcgctgcc agtttttttc atatgccacg caaacatttc
541acaaggcaga gtaccggaac aattgcctat taaagtacag tcccggagga acacctaccg
601ctataaaggt gctgagtaac gtggaatctg gattctcact gaagccctgt gccctttcag
661aaattggttg ccacatgaac atcttccagc atcttgcgtt ctcagatgtg gatgttgcca
721gggttctcac tccagatgct tttgtgtgtc ggaccatctg cacctatcac cccaactgcc
781tcttctttac attctataca aatgtatgga aaatcgagtc acaaagaaat gtttgtcttc
841ttaaaacatc tgaaagtggc acaccaagtt cctctactcc tcaagaaaac accatatctg
901gatatagcct tttaacctgc aaaagaactt tacctgaacc ctgccattct aaaatttacc
961cgggagttga ctttggagga gaagaattga atgtgacttt tgttaaagga gtgaatgttt
1021gccaagagac ttgcacaaag atgattcgct gtcagttttt cacttattct ttactcccag
1081aagactgtaa ggaagagaag tgtaagtgtt tcttaagatt atctatggat ggttctccaa
1141ctaggattgc gtatgggaca caagggagct ctggttactc tttgagattg tgtaacactg
1201gggacaactc tgtctgcaca acaaaaacaa gcacacgcat tgttggagga acaaactctt
1261cttggggaga gtggccctgg caggtgagcc tgcaggtgaa gctgacagct cagaggcacc
1321tgtgtggagg gtcactcata ggacaccagt gggtcctcac tgctgcccac tgctttgatg
1381ggcttcccct gcaggatgtt tggcgcatct atagtggcat tttaaatctg tcagacatta
1441caaaagatac acctttctca caaataaaag agattattat tcaccaaaac tataaagtct
1501cagaagggaa tcatgatatc gccttgataa aactccaggc tcctttgaat tacactgaat
1561tccaaaaaCC AATATGCCTA CCTTCCAAAG GTGACACAAG CACAATTTAT ACCAACTGTT
1621GGGTAACCGG ATGGGGCTTC TCGAAGGAGA AAGGTGAAAT CCAAAATATT CTACAAAAGG
1681TAAATATTCC TTTGGTAACA AATGAAGAAT GCCAGAAAAG ATATCAAGAT TATAAAATAA
1741CCCAACGGAT GGTCTGTGCT GGCTATAAAG AAGGGGGAAA AGATGCTTGT AAGGGAGATT
1801CAGGTGGTCC CTTAGTTTGC AAACACAATG GAATGTGGCG TTTGGTGGGC ATCACCAGCT
1861GGGGTGAAGG CTGTGCCCGC AGGGAGCAAC CTGGTGTCTA CACCAAAGTC GCTGAGTACA
1921TGGACTGGAT TTTAGAGAAA ACACAGAGCA GTGATGGAAA AGCTCAGATG CAGTCACCAG
1981CATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
2041TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
2101GGCTTTATAT ATCTTGTGGA AAGGACGACC AGACGATTGT AAGTCAATAG ATACGCGTTA
2161AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)