Transcript: Human XM_017008184.1

PREDICTED: Homo sapiens kallikrein B1 (KLKB1), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
KLKB1 (3818)
Length:
1702
CDS:
127..1437

Additional Resources:

NCBI RefSeq record:
XM_017008184.1
NBCI Gene record:
KLKB1 (3818)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017008184.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000422214 AGGATGTTTGGCGCATCTATA pLKO_005 845 CDS 100% 13.200 10.560 N KLKB1 n/a
2 TRCN0000412526 ATACACCTTTCTCACAAATAA pLKO_005 899 CDS 100% 15.000 10.500 N KLKB1 n/a
3 TRCN0000050642 GCCTCTTCTTTACATTCTATA pLKO.1 199 CDS 100% 13.200 9.240 N KLKB1 n/a
4 TRCN0000050638 GCTGAGTACATGGACTGGATT pLKO.1 1363 CDS 100% 4.950 3.465 N KLKB1 n/a
5 TRCN0000431578 CAACCTGAGTTCAAGTCAAAT pLKO_005 1467 3UTR 100% 13.200 7.920 N KLKB1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017008184.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06493 pDONR223 100% 65.6% 61.8% None 0_1ins637;114_144del n/a
2 ccsbBroad304_06493 pLX_304 0% 65.6% 61.8% V5 0_1ins637;114_144del n/a
3 TRCN0000472800 CCAGACGATTGTAAGTCAATAGAT pLX_317 21.9% 65.6% 61.8% V5 0_1ins637;114_144del n/a
4 ccsbBroadEn_06492 pDONR223 100% 65.5% 61.6% None (many diffs) n/a
5 ccsbBroad304_06492 pLX_304 0% 65.5% 61.6% V5 (many diffs) n/a
6 TRCN0000474227 TACTGTATTCCGACACTGACTGTC pLX_317 19.1% 65.5% 61.6% V5 (many diffs) n/a
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