Construct: ORF TRCN0000472802
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003513.1_s317c1
- Derived from:
- ccsbBroadEn_02478
- DNA Barcode:
- ACAAATTCCAATAGAATATCATGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAB21L2 (10586)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472802
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10586 | MAB21L2 | mab-21 like 2 | NM_006439.5 | 100% | 100% | |
| 2 | human | 4081 | MAB21L1 | mab-21 like 1 | NM_005584.5 | 79.6% | 94.1% | (many diffs) |
| 3 | mouse | 23937 | Mab21l2 | mab-21-like 2 | NM_011839.4 | 92.3% | 99.7% | (many diffs) |
| 4 | mouse | 17116 | Mab21l1 | mab-21-like 1 (C. elegans) | NM_010750.3 | 80.5% | 94.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1143
- ORF length:
- 1077
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat cgccgctcag gccaagctgg tttaccagct caataagtac tacactgagc 121 gctgtcaggc gcgcaaggcg gccatcgcca aaaccatccg agaggtctgt aaggtggtct 181 cggacgtgct caaggaagtg gaggtgcagg agcctcgctt catcagctcc ttgagcgaga 241 tcgatgcccg ctacgagggg ctcgaggtca tttcgcccac cgaatttgag gtggtgctct 301 acctaaacca gatgggcgtc ttcaacttcg tggacgacgg ctcgctgccc ggctgcgcag 361 tgctcaaact gagcgatggg cggaagcgga gcatgtctct ctgggtcgag ttcatcacgg 421 cgtcgggcta tctctcagcg cgtaagatcc gctcgcgttt ccagacgctg gtggcccagg 481 cggtggacaa gtgcagctat cgggatgtgg tcaagatgat cgcggacacc agcgaggtca 541 agttgcgcat cagggagcgc tatgtggtgc aaatcactcc ggcgttcaag tgcaccggga 601 tctggcctcg cagcgcggca cagtggccta tgccccacat cccttggccc ggccccaatc 661 gggtggccga ggtcaaggcc gaagggttca acttgctctc gaaggagtgc tactcgctga 721 ccggcaagca gagctcggca gagagcgacg cctgggtgct acagttcggg gaggcggaga 781 accgcctgct gatgggcggc tgccgaaaca agtgcctctc agtgctgaag actctgcggg 841 accgccacct ggagctaccc ggccagccgc tcaacaacta ccacatgaag acgctgcTGC 901 TGTACGAGTG CGAGAAACAC CCACGAGAAA CGGACTGGGA CGAGTCGTGC CTGGGCGACC 961 GGCTCAACGG CATCCTGCTG CAGCTCATCT CCTGCCTGCA GTGCCGCCGC TGCCCTCACT 1021 ACTTTCTGCC CAACCTCGAC CTCTTTCAGG GCAAGCCCCA TTCGGCCCTG GAGAGCGCTG 1081 CCAAGCAGAC CTGGAGGTTG GCCAGGGAAA TTCTCACCAA TCCCAAAAGC CTGGACAAAC 1141 TATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGAAC AAATTCCAAT AGAATATCAT GGACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt