Construct: ORF TRCN0000472809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003902.1_s317c1
- Derived from:
- ccsbBroadEn_00781
- DNA Barcode:
- CTTCGACAGGAACCACACGTTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HPD (3242)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3242 | HPD | 4-hydroxyphenylpyruvate dio... | NM_002150.3 | 99.9% | 99.7% | 97A>G |
| 2 | human | 3242 | HPD | 4-hydroxyphenylpyruvate dio... | NM_001171993.1 | 90% | 90% | 0_1ins117 |
| 3 | mouse | 15445 | Hpd | 4-hydroxyphenylpyruvic acid... | NM_008277.3 | 86% | 89.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1245
- ORF length:
- 1179
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gacttacagt gacaaagggg caaagcctga gagaggccga ttcctccact 121 tccactctgt gaccttctgg gttggcaacg ccaagcaggc cgcgtcattc tactgcagca 181 agatgggctt tgaacctcta gcctacaggg gcctggagac cggttcccgg gaggtggtca 241 gccatgtaat caaacaaggg aagattgtgt ttgtcctctc ctcagcgctc aacccctgga 301 acaaagagat gggcgatcac ctggtgaaac acggtgacgg agtgaaggac attgcgttcg 361 aggtggaaga ttgtgactac atcgtgcaga aagcacggga acggggcgcc aaaatcatgc 421 gggagccctg ggtagagcaa gacaagtttg ggaaggtgaa gtttgctgtg ctgcagacgt 481 atggggacac cacacacacc ctggtggaga agatgaacta catcggccaa ttcttgcctg 541 gatatgaggc cccagcgttc atggaccccc tacttcctaa actgcccaaa tgcagtctgg 601 agatgatcga ccacattgtg ggaaaccagc ctgatcagga gatggtgtcc gcctccgaat 661 ggtacctgaa aaacctgcag ttccaccgct tctggtccgt ggatgacacg caggtgcaca 721 cggaatatag ctctctgcga tccattgtgg tggccaacta tgaagagtcc atcaagatgc 781 ccatcaatga gccagcgcct ggcaagaaga agtcccagat ccaggaatat gtggactata 841 acgggggcgc tggGGTCCAG CACATCGCTC TCAAGACCGA AGACATCATC ACAGCGATTC 901 GCCACTTGAG AGAGAGAGGC CTGGAGTTCT TATCTGTTCC CTCCACGTAC TACAAACAAC 961 TGCGGGAGAA GCTGAAGACG GCCAAGATCA AGGTGAAGGA GAACATTGAT GCCCTGGAGG 1021 AGCTGAAAAT CCTGGTGGAC TACGACGAGA AAGGCTACCT CCTGCAGATC TTCACCAAAC 1081 CGGTGCAGGA CCGGCCCACG CTCTTCCTGG AAGTCATCCA GCGCCACAAC CACCAGGGTT 1141 TTGGAGCCGG CAACTTCAAC TCACTGTTCA AGGCTTTCGA GGAGGAGCAG AACCTGCGGG 1201 GTAACCTCAC CAACATGGAG ACCAATGGGG TGGTGCCCGG CATGTACCCA ACTTTCTTGT 1261 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1321 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1381 GAAAGGACGA CTTCGACAGG AACCACACGT TATTACGCGT TAAGTCgaca atcaacctct 1441 ggattacaaa atttgtgaaa gatt