Construct: ORF TRCN0000472811
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006979.1_s317c1
- Derived from:
- ccsbBroadEn_01939
- DNA Barcode:
- CCGTGGCAACTTCTTCCTGTGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RGS5 (8490)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472811
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8490 | RGS5 | regulator of G protein sign... | NM_003617.4 | 100% | 100% | |
| 2 | human | 8490 | RGS5 | regulator of G protein sign... | NM_001254749.2 | 97.8% | 97.8% | 384_395del |
| 3 | human | 8490 | RGS5 | regulator of G protein sign... | NM_001195303.3 | 40.3% | 40.3% | 0_1ins324 |
| 4 | human | 8490 | RGS5 | regulator of G protein sign... | NM_001254748.1 | 40.3% | 40.3% | 0_1ins324 |
| 5 | mouse | 19737 | Rgs5 | regulator of G-protein sign... | NM_009063.4 | 89.8% | 90% | (many diffs) |
| 6 | mouse | 19737 | Rgs5 | regulator of G-protein sign... | NM_001313705.1 | 35.7% | 34.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg caaaggactt gcagctttgc cccactcatg cctggaaagg gccaaggaga 121 ttaagatcaa gttgggaatt ctcctccaga agccagacTC AGTTGGTGAC CTTGTCATTC 181 CGTACAATGA GAAGCCAGAG AAACCAGCCA AGACCCAGAA AACCTCGCTG GACGAGGCCC 241 TGCAGTGGCG TGATTCCCTG GACAAACTCC TGCAGAACAA CTATGGACTT GCCAGTTTCA 301 AAAGTTTCCT GAAGTCTGAA TTCAGTGAGG AAAACCTTGA GTTCTGGATT GCCTGTGAGG 361 ATTACAAGAA GATCAAGTCC CCTGCCAAGA TGGCTGAGAA GGCAAAGCAA ATTTATGAAG 421 AATTCATTCA AACGGAGGCT CCTAAAGAGG TGAATATTGA CCACTTCACT AAGGACATCA 481 CAATGAAGAA CCTGGTGGAA CCTTCCCTGA GCAGCTTTGA CATGGCCCAG AAAAGAATCC 541 ATGCCCTGAT GGAAAAGGAT TCTCTGCCTC GCTTTGTGCG CTCTGAGTTT TATCAGGAGT 601 TAATCAAGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCGTGG CAACTTCTTC CTGTGAAAAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt