Construct: ORF TRCN0000472812

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013221.1_s317c1
Derived from:
ccsbBroadEn_03298
DNA Barcode:
GTGGTAAACTACAGTGAATACGAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AIG1 (51390)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472812
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51390 AIG1 androgen induced 1 NM_016108.4 100% 100%
2 human 51390 AIG1 androgen induced 1 NM_001366350.1 98.7% 98.3% 679_680insGTATGGAAG
3 human 51390 AIG1 androgen induced 1 NM_001366351.1 98% 97% 4A>G;7_7delAinsCTTGTCCCCTGCC
4 human 51390 AIG1 androgen induced 1 NM_001366348.1 97.5% 97.1% 680_697del
5 human 51390 AIG1 androgen induced 1 NM_001363721.2 96.3% 94.9% (many diffs)
6 human 51390 AIG1 androgen induced 1 NM_001286587.1 95.7% 95.7% 0_1ins30
7 human 51390 AIG1 androgen induced 1 NM_001366349.2 95.3% 92.6% (many diffs)
8 human 51390 AIG1 androgen induced 1 NM_001366347.1 94.6% 90.4% (many diffs)
9 human 51390 AIG1 androgen induced 1 NM_001366346.1 91.8% 92.7% (many diffs)
10 human 51390 AIG1 androgen induced 1 NM_001366345.1 90% 88.9% (many diffs)
11 human 51390 AIG1 androgen induced 1 NM_001366353.1 85.7% 85.7% 297_298ins102
12 human 51390 AIG1 androgen induced 1 NM_001366344.1 83.5% 83.5% 141_281del
13 human 51390 AIG1 androgen induced 1 NM_001366354.1 82.3% 62.5% 396_397ins116;588_589insTAAATTGGAA
14 human 51390 AIG1 androgen induced 1 NM_001366355.1 78.1% 78.1% 141_142ins156
15 human 51390 AIG1 androgen induced 1 NM_001366357.1 74.1% 73.1% (many diffs)
16 human 51390 AIG1 androgen induced 1 NM_001366352.1 74% 55% (many diffs)
17 human 51390 AIG1 androgen induced 1 NM_001366358.1 63% 63% 0_1ins264
18 human 51390 AIG1 androgen induced 1 NM_001286589.1 56.6% 56.3% (many diffs)
19 human 51390 AIG1 androgen induced 1 NM_001286588.2 56% 55.8% 401delT;402_403ins313
20 human 51390 AIG1 androgen induced 1 NM_001366359.1 54.6% 53.3% (many diffs)
21 human 51390 AIG1 androgen induced 1 NM_001366361.1 48.4% 45% (many diffs)
22 human 51390 AIG1 androgen induced 1 NM_001366356.1 47.3% 47% (many diffs)
23 human 51390 AIG1 androgen induced 1 NR_158960.1 28.7% (many diffs)
24 human 51390 AIG1 androgen induced 1 NR_158896.1 27% (many diffs)
25 human 51390 AIG1 androgen induced 1 NM_001366363.1 19.7% 19.7% 141_142ins573
26 human 51390 AIG1 androgen induced 1 NM_001366362.1 17.1% 16% (many diffs)
27 mouse 66253 Aig1 androgen-induced 1 NM_001347362.1 89.2% 95.3% (many diffs)
28 mouse 66253 Aig1 androgen-induced 1 NM_025446.4 79.8% 81.2% (many diffs)
29 mouse 66253 Aig1 androgen-induced 1 XM_006512812.3 62.9% 62.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
780
ORF length:
714
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gcttgtcccc tgccaggtgc tgcggatggc aatcctgctg tcttactgct
121ctatcctgtg taactacaag gccatcgaaa tgccctcaca ccagacctac ggagggagct
181ggaaattcct gacgttcatt gatctggtta tccaggctgt cttttttggc atctgtgtgc
241tgactgatct ttccagtctt ctgactcgag gaagtgggaa ccaggagcaa gagaggcagc
301tcaagaagct catctctctc cgggactgga tgttagctgt gttggccttt cctgttgggg
361tttttgttgt agcagtgttc tggatcattt atgcctatga cagagagatg atatacccga
421agctgctgga taattttatc ccagggtggc tgaaTCACGG AATGCACACG ACGGTTCTGC
481CCTTTATATT AATCGAGATG AGGACATCGC ACCATCAGTA TCCCAGCAGG AGCAGCGGAC
541TTACCGCCAT ATGTACCTTC TCTGTTGGCT ATATATTATG GGTGTGCTGG GTGCATCATG
601TAACTGGCAT GTGGGTGTAC CCTTTCCTGG AACACATTGG CCCAGGAGCC AGAATCATCT
661TCTTTGGGTC TACAACCATC TTAATGAACT TCCTGTACCT GCTGGGAGAA GTTCTGAACA
721ACTATATCTG GGATACACAG AAAAGTATGG AAGAAGAGAA AGAAAAGCCT AAATTGGAAT
781GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG
841GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
901TTTATATATC TTGTGGAAAG GACGAGTGGT AAACTACAGT GAATACGAGA CGCGTTAAGT
961Cgacaatcaa cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)