Construct: ORF TRCN0000472813
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010170.1_s317c1
- Derived from:
- ccsbBroadEn_02718
- DNA Barcode:
- CAAGGACCGATATGTAAGTTGTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERP44 (23071)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472813
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23071 | ERP44 | endoplasmic reticulum prote... | NM_015051.3 | 100% | 100% | |
| 2 | mouse | 76299 | Erp44 | endoplasmic reticulum prote... | NM_029572.2 | 89.9% | 93.1% | (many diffs) |
| 3 | mouse | 76299 | Erp44 | endoplasmic reticulum prote... | XM_006538341.3 | 68.9% | 72.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1284
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca tcctgccgtc ttcctatcct tacccgacct cagatgctcc cttctgctcc 121 tggtaacttg ggtttttact cctgtaacaa ctgaaataac aagtcttgat acagagaata 181 tagatgaaat tttaaacaat gctgatgttg ctttagtaaa tttttatgct gactggtgtc 241 gtttcagtca gatgttgcat ccaatttttg aggaagcttc cgatgtcatt aaggaagaat 301 ttccaaatga aaatcaagta gtgtttgcca gagttgattg tgatcagcac tctgacatag 361 cccagagata caggataagc aaatacccaa ccctcaaatt gtttcgtaat gggatgatga 421 tgaagagaga atacaggggt cagcgatcag tgaaagcatt ggcagattac atcaggcaac 481 aaaaaagtga ccccattcaa gaaattcggg acttagcaga aatcaccact cttgatcgca 541 gcaaaagaaa tatcattgga tattttgagc aaaaggactc ggacaactat agagtttttg 601 aacgagtagc gaatattttg catgatgact gtgcctttct ttctgcattt ggggatgttt 661 caaaaccgga aagatatagt ggcgacaaca taatctacaa accaccaggg cattctgctc 721 cggatatggt gtacttggga gctatgacaa attttgatgt gacttacaat tggattcaag 781 ataaatgtgt tcctcttgtc cgagaaataa catttgaaaa tggagaggaa ttgacagaag 841 aaggactgCC TTTTCTCATA CTCTTTCACA TGAAAGAAGA TACAGAAAGT TTAGAAATAT 901 TCCAGAATGA AGTAGCTCGG CAATTAATAA GTGAAAAAGG TACAATAAAC TTTTTACATG 961 CCGATTGTGA CAAATTTAGA CATCCTCTTC TGCACATACA GAAAACTCCA GCAGATTGTC 1021 CTGTAATCGC TATTGACAGC TTTAGGCATA TGTATGTGTT TGGAGACTTC AAAGATGTAT 1081 TAATTCCTGG AAAACTCAAG CAATTCGTAT TTGACTTACA TTCTGGAAAA CTGCACAGAG 1141 AATTCCATCA TGGACCTGAC CCAACTGATA CAGCCCCAGG AGAGCAAGCC CAAGATGTAG 1201 CAAGCAGTCC ACCTGAGAGC TCCTTCCAGA AACTAGCACC CAGTGAATAT AGGTATACTC 1261 TATTGAGGGA TCGAGATGAG CTTTACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1321 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1381 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC AAGGACCGAT 1441 ATGTAAGTTG TCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1501 att