Construct: ORF TRCN0000472814
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010092.1_s317c1
- Derived from:
- ccsbBroadEn_10727
- DNA Barcode:
- GGACATTGCTGCCATTCTGAACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CDKN2A (1029)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472814
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1029 | CDKN2A | cyclin dependent kinase inh... | NM_001363763.2 | 100% | 100% | |
| 2 | human | 1029 | CDKN2A | cyclin dependent kinase inh... | XM_005251343.1 | 100% | 100% | |
| 3 | human | 1029 | CDKN2A | cyclin dependent kinase inh... | NM_000077.4 | 67.3% | 67.3% | 1_153del |
| 4 | human | 1029 | CDKN2A | cyclin dependent kinase inh... | NM_001195132.1 | 61.3% | 60.4% | (many diffs) |
| 5 | human | 1029 | CDKN2A | cyclin dependent kinase inh... | XM_011517676.2 | 60.4% | 59.3% | (many diffs) |
| 6 | human | 1029 | CDKN2A | cyclin dependent kinase inh... | XM_011517675.2 | 56.7% | 55.4% | (many diffs) |
| 7 | human | 1030 | CDKN2B | cyclin dependent kinase inh... | NM_004936.4 | 49.7% | 50.6% | (many diffs) |
| 8 | human | 1029 | CDKN2A | cyclin dependent kinase inh... | NM_058195.3 | 39.1% | 18.2% | 1_196del;396_397ins115 |
| 9 | human | 1029 | CDKN2A | cyclin dependent kinase inh... | XR_929159.2 | 12% | (many diffs) | |
| 10 | mouse | 12579 | Cdkn2b | cyclin-dependent kinase inh... | NM_007670.4 | 48% | 52% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 381
- ORF length:
- 315
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gatgggcagc gcccgagtgg cggagctgcT GCTGCTCCAC GGCGCGGAGC 121 CCAACTGCGC CGACCCCGCC ACTCTCACCC GACCCGTGCA CGACGCTGCC CGGGAGGGCT 181 TCCTGGACAC GCTGGTGGTG CTGCACCGGG CCGGGGCGCG GCTGGACGTG CGCGATGCCT 241 GGGGCCGTCT GCCCGTGGAC CTGGCTGAGG AGCTGGGCCA TCGCGATGTC GCACGGTACC 301 TGCGCGCGGC TGCGGGGGGC ACCAGAGGCA GTAACCATGC CCGCATAGAT GCCGCGGAAG 361 GTCCCTCAGA CATCCCCGAT AGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGAC ATTGCTGCCA 541 TTCTGAACGC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt