Construct: ORF TRCN0000472825
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002034.1_s317c1
- Derived from:
- ccsbBroadEn_14292
- DNA Barcode:
- TAACTTTTCGTTTCAACGCGCCAG
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VMP1 (81671)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472825
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81671 | VMP1 | vacuole membrane protein 1 | NM_001329394.2 | 99.9% | 99.2% | 1204delG |
| 2 | human | 81671 | VMP1 | vacuole membrane protein 1 | NM_030938.5 | 99.9% | 99.2% | 1204delG |
| 3 | human | 81671 | VMP1 | vacuole membrane protein 1 | NM_001329395.2 | 96.8% | 96.1% | 974_1012del;1243delG |
| 4 | human | 81671 | VMP1 | vacuole membrane protein 1 | NM_001329396.2 | 90.8% | 90.1% | 302_303ins111;1093delG |
| 5 | human | 81671 | VMP1 | vacuole membrane protein 1 | NM_001329397.2 | 86.1% | 85.4% | 413_414ins168;1036delG |
| 6 | human | 81671 | VMP1 | vacuole membrane protein 1 | NM_001329399.2 | 75.7% | 74.3% | (many diffs) |
| 7 | human | 81671 | VMP1 | vacuole membrane protein 1 | NM_001329398.2 | 73.4% | 72% | (many diffs) |
| 8 | human | 81671 | VMP1 | vacuole membrane protein 1 | NM_001329400.2 | 65.8% | 65.2% | (many diffs) |
| 9 | human | 81671 | VMP1 | vacuole membrane protein 1 | NM_001329401.2 | 61.9% | 60.5% | (many diffs) |
| 10 | human | 81671 | VMP1 | vacuole membrane protein 1 | NM_001329402.2 | 52.6% | 51.9% | 0_1ins576;628delG |
| 11 | mouse | 75909 | Vmp1 | vacuole membrane protein 1 | NM_029478.3 | 90.9% | 93.3% | (many diffs) |
| 12 | mouse | 75909 | Vmp1 | vacuole membrane protein 1 | XM_006534403.3 | 90.9% | 93.3% | (many diffs) |
| 13 | mouse | 75909 | Vmp1 | vacuole membrane protein 1 | XM_006534402.2 | 87.1% | 89.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1284
- ORF length:
- 1218
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agagaatgga aaaaattgtg accagagacg tgtagcaatg aacaaggaac 121 atcataatgg aaatttcaca gacccctctt cagtgaatga aaagaagagg agggagcggg 181 aagaaaggca gaatattgtc ctgtggagac agccgctcat taccttgcag tatttttctc 241 tggaaatcct tgtaatcttg aaggaatgga cctcaaaatt atggcatcgt caaagcattg 301 tggtgtcttt tttactgctg cttgctgtgc ttatagctac gtattatgtt gaaggagtgc 361 atcaacagta tgtgcaacgt atagagaaac agtttctttt gtatgcctac tggataggct 421 taggaatttt gtcttctgtt gggcttggaa cagggctgca cacctttctg ctttatctgg 481 gtccacatat agcctcagtt acattagctg cttatgaatg caattcagtt aattttcccg 541 aaccacccta tcctgatcag attatttgtc cagatgaaga gggcactgaa ggaaccattt 601 ctttgtggag tatcatctca aaagttagga ttgaagcctg catgtggggt atcggtacag 661 caatcggaga gctgcctcca tatttcatgg ccagagcagc tcgcctctca ggtgctgaac 721 cagatgatga agagtatcag gaatttgaag agatgctgga acatgcagag tctgcacaag 781 actttgcctc ccgggccaaa ctggcagttc aaaaactagt acagaaagtt ggatttTTTG 841 GAATTTTGGC CTGTGCTTCA ATTCCAAATC CTTTATTTGA TCTGGCTGGA ATAACGTGTG 901 GACACTTTCT GGTACCTTTT TGGACCTTCT TTGGTGCAAC CCTAATTGGA AAAGCAATAA 961 TAAAAATGCA TATCCAGAAA ATTTTTGTTA TAATAACATT CAGCAAGCAC ATAGTGGAGC 1021 AAATGGTGGC TTTCATTGGT GCTGTCCCCG GCATAGGTCC ATCTCTGCAG AAGCCATTTC 1081 AGGAGTACCT GGAGGCTCAA CGGCAGAAGC TTCACCACAA AAGCGAAATG GGCACACCAC 1141 AGGGAGAAAA CTGGTTGTCC TGGATGTTTG AAAAGTTGGT CGTTGTCATG GTGTGTTACT 1201 TCATCCTATC TATCATTAAC TCCATGGCAC AAAGTTATGC CAAACGAATC CAGCAGCGGT 1261 TGAACTCAAG GAGAAAACTA AAGACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 1321 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 1381 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA ACTTTTCGTT 1441 TCAACGCGCC AGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 1501 tt