Construct: ORF TRCN0000472828
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016776.1_s317c1
- Derived from:
- ccsbBroadEn_01607
- DNA Barcode:
- GCACTTCAACGCACACGCCCATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAT1 (6772)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472828
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6772 | STAT1 | signal transducer and activ... | NM_139266.2 | 100% | 100% | |
| 2 | human | 6772 | STAT1 | signal transducer and activ... | NM_007315.3 | 94.8% | 94.9% | 2136_2250delinsG |
| 3 | human | 6772 | STAT1 | signal transducer and activ... | XM_006712718.1 | 94.8% | 94.9% | 2136_2250delinsG |
| 4 | human | 6772 | STAT1 | signal transducer and activ... | XM_017004783.2 | 94.6% | 94.6% | 1_6delATGTGG;2142_2256delinsG |
| 5 | human | 6772 | STAT1 | signal transducer and activ... | XR_001738915.2 | 44.4% | 1_335del;2472_4804del | |
| 6 | human | 6772 | STAT1 | signal transducer and activ... | XR_001738914.2 | 43.9% | 1_393del;2530_4862del | |
| 7 | mouse | 20846 | Stat1 | signal transducer and activ... | NM_001205314.1 | 82.2% | 89% | (many diffs) |
| 8 | mouse | 20846 | Stat1 | signal transducer and activ... | NM_009283.4 | 82.2% | 89% | (many diffs) |
| 9 | mouse | 20846 | Stat1 | signal transducer and activ... | XM_006495811.3 | 82.2% | 89% | (many diffs) |
| 10 | mouse | 20846 | Stat1 | signal transducer and activ... | XM_017319463.1 | 82.2% | 89% | (many diffs) |
| 11 | mouse | 20846 | Stat1 | signal transducer and activ... | XM_017319464.1 | 82.2% | 89% | (many diffs) |
| 12 | mouse | 20846 | Stat1 | signal transducer and activ... | NM_001205313.1 | 81.5% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2202
- ORF length:
- 2136
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcagtggtac gaacttcagc agcttgactc aaaattcctg gagcaggttc 121 accagcttta tgatgacagt tttcccatgg aaatcagaca gtacctggca cagtggttag 181 aaaagcaaga ctgggagcac gctgccaatg atgtttcatt tgccaccatc cgttttcatg 241 acctcctgtc acagctggat gatcaatata gtcgcttttc tttggagaat aacttcttgc 301 tacagcataa cataaggaaa agcaagcgta atcttcagga taattttcag gaagacccaa 361 tccagatgtc tatgatcatt tacagctgtc tgaaggaaga aaggaaaatt ctggaaaacg 421 cccagagatt taatcaggct cagtcgggga atattcagag cacagtgatg ttagacaaac 481 agaaagagct tgacagtaaa gtcagaaatg tgaaggacaa ggttatgtgt atagagcatg 541 aaatcaagag cctggaagat ttacaagatg aatatgactt caaatgcaaa accttgcaga 601 acagagaaca cgagaccaat ggtgtggcaa agagtgatca gaaacaagaa cagctgttac 661 tcaagaagat gtatttaatg cttgacaata agagaaagga agtagttcac aaaataatag 721 agttgctgaa tgtcactgaa cttacccaga atgccctgat taatgatgaa ctagtggagt 781 ggaagcggag acagcagagc gcctgtattg gggggccgcc caatgcttgc ttggatcagc 841 tgcagaactg gttcactata gttgcggaga gtctgcagca agttcggcag cagcttaaaa 901 agttggagga attggaacag aaatacacct acgaacatga ccctatcaca aaaaacaaac 961 aagtgttatg ggaccgcacc ttcagtcttt tccagcagct cattcagagc tcgtttgtgg 1021 tggaaagaca gccctgcatg ccaacgcacc ctcagaggcc gctggtcttg aagacagggg 1081 tccagttcac tgtgaagttg agactgttgg tgaaattgca agagctgaat tataatttga 1141 aagtcaaagt cttatttgat aaagatgtga atgagagaaa tacagtaaaa ggatttagga 1201 agttcaacat tttgggcacg cacacaaaag tgatgaacat ggaggagtcc accaatggca 1261 gtctggcggc tgaatttcgg cacctgcaat tgaaagaaca gaaaaatgct ggcaccagaa 1321 cgaatgaggg tcctctcatc gttactgaag agcttcactc ccttagtttt gaaacccaat 1381 tgtgccagcc tggtttggta attgacctcg agacgacctc tctgcccgtt gtggtgatct 1441 ccaacgtcag ccagctcccg agcggttggg cctccatcct ttggtacaac atgctggtgg 1501 cggaacccag gaatctgtcc ttcttcctga ctccaccatg tgcacgatgg gctcagcttt 1561 cagaagtgct gagttggcag ttttcttctg tcaccaaaag aggtctcaat gtggaccagc 1621 tgaacatgtt gggagagaag cttcttggtc ctaacgccag ccccgatggt ctcattccgt 1681 ggacgaggtt ttgtaaggaa aatataaatg ataaaaattt tcccttctgg ctttggattg 1741 aaagcatcct agaactcatt aaaaaacacc tgctccctct ctggaatgat gggtgcatca 1801 tgggcttcat cagcaaggag cgagagcgtg ccctgttgaa ggaccagcag ccggggacct 1861 tcctgctgcg gttcagtgag agctcccggg aaggggccat cacatTCACA TGGGTGGAGC 1921 GGTCCCAGAA CGGAGGCGAA CCTGACTTCC ATGCGGTTGA ACCCTACACG AAGAAAGAAC 1981 TTTCTGCTGT TACTTTCCCT GACATCATTC GCAATTACAA AGTCATGGCT GCTGAGAATA 2041 TTCCTGAGAA TCCCCTGAAG TATCTGTATC CAAATATTGA CAAAGACCAT GCCTTTGGAA 2101 AGTATTACTC CAGGCCAAAG GAAGCACCAG AGCCAATGGA ACTTGATGGC CCTAAAGGAA 2161 CTGGATATAT CAAGACTGAG TTGATTTCTG TGTCTGAAGT GTACCCAACT TTCTTGTACA 2221 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2281 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2341 AGGACGAGCA CTTCAACGCA CACGCCCATT CACGCGTTAA GTCgacaatc aacctctgga 2401 ttacaaaatt tgtgaaagat t